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Safety Information

SCCE016

Sigma-Aldrich

Human Corneal Epithelial Cells

Human Corneal Epithelial Cells provide an ideal serum-free culture model without antimicrobials or phenol red when used with the EpiGRO Ocular Epithelia Complete Media Kit.

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Synonym(s):
HCEC cells
UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.71

biological source

human cornea (epithelial cells)

Quality Level

packaging

vial of 500,000 cells

manufacturer/tradename

Chemicon®
EpiGRO®
Millipore

morphology

(epithelial)

technique(s)

cell culture | mammalian: suitable

shipped in

liquid nitrogen

General description

Human Corneal Epithelial Cells may be used for studies of cell-matrix interactions, gene regulation and tissue development, drug development, and validation of alternative methods in toxicology. The Human Corneal Epithelial Cells are cryopreserved as secondary cells. The cells have been isolated from human corneal tissue and expanded twice in culture vessels before being harvested for cryopreservation. This product is for Research Use Only. This product is not approved for human or veterinary use, or for use in in vitro diagnostic or clinical procedures.
Product Source: Human Corneal Epithelia

Cell Line Origin

Cornea

Cell Line Description

Epithelial Cells

Application

Please see data sheet for detailed directions on Thawing, Plating , Feeding, Passaging, etc.
Research Category
Stem Cell Research

Packaging

500,000 cells

Components

SCCE016

Quality

Cells are negative for HIV (1,2), HBV, HCV, bacteria, mycoplasma and fungal contamination.

Further growth: Guaranteed to provide 3 or more passages when grown in the appropriate EpiGRO Medium.

Storage and Stability

To maintain the cells’ integrity, Millipore recommends unpacking the products immediately upon receipt and storing at a temperature lower than -150°C or in liquid nitrogen vapor phase.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
EPIGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

高风险级别生物产品--人源产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Wei Yang Seow et al.
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Aung Than et al.
Nature communications, 9(1), 4433-4433 (2018-11-08)
Eye diseases and injuries impose a significant clinical problem worldwide. Safe and effective ocular drug delivery is, however, challenging due to the presence of ocular barriers. Here we report a strategy using an eye patch equipped with an array of
Adrian Gericke et al.
RSC advances, 9(39), 22531-22539 (2019-07-22)
Impaired regeneration of the corneal epithelium, as found in many ocular surface diseases, is a major clinical problem in ophthalmology. We hypothesized that corneal epithelial regeneration can be promoted by the physiological, energy-delivering as well as "morphogenetically active" polymer, inorganic
Qiaojuan Zhang et al.
Journal of neurochemistry, 151(2), 238-254 (2019-03-20)
Herpes simplex virus-type 1 (HSV-1) infection of sensory neurons may lead to a significant reduction in the expression of voltage-activated Na+ and Ca2+ channels, which can disrupt the transmission of pain information. Viral infection also results in the secretion of

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