SCC300
3dGRO® Human iPSC Derived Colon Organoids
Normal, Dermal Fibroblast, Age: Neonatal, Male, Mutation: None
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About This Item
biological source
human (dermal fibroblast)
form
liquid
packaging
vial of >1500 viable organoids
gender
male
technique(s)
cell culture: suitable
application(s)
3D cell culture
control
(dermal fibroblast)
relevant disease(s)
(Normal)
storage temp.
−196°C
General description
3dGRO® Human iPSC Derived Colon Organoids are derived from integration-free human iPS cells (Cat. No. SCC271) reprogrammed using the Simplicon RNA reprogramming technology (Cat. No. SCR550). Human iPS cells were differentiated using a multi-step process from definitive endoderm to posterior hind-gut endoderm and eventually into colon organoids (Figure 1.). The organoids express colon-specific markers including the posterior hindgut marker CDX2, a-carbonic anydrase II (CA-II), a-carbonic anhydrase IV (CA-IV), and goblet cell markers Mucin-2 and Mucin-5B. 3dGRO® human iPSC derived colon organoids can be propagated long-term by passaging every 10-12 days using 3dGRO® Human Colon Organoid Expansion Medium (Cat. No. SCM304).
Preparation Note
Analysis Note
- Each vial contains ≥1500 organoids.
- Mycoplasma: Negative
- Genotyped by STR analysis: Pass
- Human species verified
- Organoids are negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
- Cell viability test: Pass
Legal Information
3dGRO® organoids were derived utilizing HUB Organoid Technology.
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/pdouse. This product may not be re-engineered or copied; used to make derivatives or modifications; used to develop, test, or manufacture a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact organoids@milliporesigma.com.
Disclaimer
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Regulatory Information
This item has
Certificates of Analysis (COA)
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Protocols
Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.
高度表征的冻存人结肠类器官，iPS细胞分化为肠道上皮类器官的分步方案。
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| SCC300 | 04054839989728 |