U2OS EGFP-H2B Human Osteosarcoma Cell Line

Chromosomal abnormalities underlie a substantial proportion of cancers, including most solid tumors (1). Chromosome instability manifests in a range of defects, from aneuploidy (abnormal number of entire chromosomes), to chromosomal breakage and rearrange

Chromosomal abnormalities underlie a substantial proportion of cancers, including the majority of solid tumors.1 Chromosome instability manifests in a range of defects, from aneuploidy (abnormal number of entire chromosomes), to chromosomal breakage and rearrangements.1 Understanding the mechanisms of chromosomal instability is key to unlocking the multifaceted roles that they play in cancer etiology and progression. The U2OS osteosarcoma is one of the first and most widely used cancer cell lines, especially for the study of mitosis, apoptosis, and the cellular mechanisms that give rise to cancer. U2OS cells undergo chromosome breakage and chromothripsis at high frequency.2 The expression of EGFP fused to a histone marker in the U2OS EGFP-H2B cell line allows for visualization of chromosome dynamics by light microscopy, a powerful tool in unraveling the complex interactions and molecular players involved in chromosomal instability.3 Expression of EGFP-H2B in this cell line is maintained via selection with blasticidin. The U2OS EGFP-H2B cell line facilitates expanded insights to chromosomal biology, allowing immediate relevancy to the many studies using the well-characterized U2OS background.Source The U2OS EGFP-H2B Human Osteosarcoma cell line was derived from U2OS cells stably transfected with a construct expressing EGFP-histone-H2B.3 The parental U2OS cell line was derived from an osteosarcoma from a 15-year-old female.2References1. Thompson SL, Compton DA. Chormosome Res. 2011; 19(3):433-444.2. Pontén J, Saksela E. Int J Cancer. 1967; 2(5):434-447.3. Wang H et al. Oncotarget 2017; 8(30):48671-48687.

Each vial contains ≥ 1X10⁶ viable cells.Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services Cells are negative for mycoplasma contamination.

U2OS EGFP-H2B Human Osteosarcoma Cell Line allows for visualization of chromosome dynamics by light microscopy

The cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

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