SAvPhire Monomeric Streptavidin peroxidase conjugate

Streptavidin, a tetrameric protein, has been extensively used in purification and detection of biomolecules. The strong streptavidin-biotin bond can highly suited for attaching various biomolecules to one another or onto a solid support. The streptavidin-biotin complex displays high resistance to organic solvents, denaturants, detergents, and proteolytic enzymes. It can also withstand extremes of temperature and pH. However, one challenge faced by researchers using the streptavidin-biotin system is the target aggregation, which results from multivalent binding. It can cause cross-linking that can interfere with observations at the molecular level. These challenges can be overcome by use of monomeric streptavidin (mSA) that displays structural and binding properties that are suitable for multiple applications based on its small size and monovalent biotin binding. The monomeric form allows for association with biotinylated ligands without inducing aggregation that are observed with multivalent interactions. Monomeric form can also be genetically fused to another protein to form a biotin-binding tag. SAvPhire Monomeric Streptavidin is a is a high-affinity recombinant variant of streptavidin that minimizes the risk of aggregation, precipitation, and crosslinking in pre-incubation steps with biotinylated molecules and offers easier access to the desired targets. Unlike other non-engineered monomeric forms, SAvPhire Monomeric Streptavidin displays highly desirable dissociation kinetics (koff =63 x 10-3 min-1 for non-engineered monomeric form and 2.1 x 10-3 min-1 for SAvPhire Monomeric Streptavidin) and longer t1/2 (11 min vs 329 min). It is suitable for multiple immunodetection applications. (Ref.: Kroestch, A., et al., (2018). Appl. Microbiol. Biotechnol., 102(23); 10079-10089).

SAvPhire Monomeric Streptavidin expressed in E. coli and conjugated with Horseradish peroxidase is suitable for immunodetection applications. SAvPhire Monomeric Streptavidin is expressed with an N-terminal His-tag and a C-terminal FLAG^® tag.

Note: Predicted to react with all species.

N/A

Immunohistochemistry (Paraffin) Analysis:

A 1:500 dilution of Anti-NeuN (ABN78) was used to stain mouse cerebral cortex tissue sections and signal was detected with Goat Anti-Rabbit IgG and SAvPhire Monomeric Streptavidin-Horseradish Peroxidase conjugate (1:100 dilution).

Evaluated by Western Blotting with Anti-GFAP-Biotin Conjugate (Cat. No. MAB3402B) and visualized with SAVphire-HRP.

Western Blotting Analysis: Mouse brain tissue lysate was probed with 1:1000 dilution of Anti-GFAP-Biotin Conjugate (Cat. No. MAB3402B) and visualized with SAvPhire Monomeric Streptavidin-HRP conjugate and chemiluminescence detection system.

Evaluated by Western Blotting with Anti-GFAP-Biotin Conjugate (Cat. No. MAB3402B) and visualized with SAVphire-HRP.

Western Blotting Analysis: Mouse brain tissue lysate was probed with 1:1000 dilution of Anti-GFAP-Biotin Conjugate (Cat. No. MAB3402B) and visualized with SAvPhire Monomeric Streptavidin-HRP conjugate and chemiluminescence detection system.

15.5 kDa for Savphire monomeric streptavidin (with an N-terminal His-tag and a C-terminal FLAG^® tag).

Format: HRP

Monomeric Streptavidin in buffer containing 0.15 M PBS, 0.5% BSA, 0.4% Bronidox, pH 7.2 without azide with 10% glycerol.

Stable for 1 year at 2-8°C from date of receipt.

Please refer to lot specific datasheet.

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.