S7705
TRAPeze™1X CHAPS Lysis Buffer
1X CHAPS Lysis Buffer is a component of the gel-and ELISA-based TRAPeze Telomerase Detection Kits & is used in the extraction step of the procedure.
Synonym(s):
CHAPS Lysis Buffer
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About This Item
UNSPSC Code:
41116012
eCl@ss:
32160405
NACRES:
NA.32
General description
1X CHAPS Lysis Buffer is a component of the gel- and ELISA-based TRAPeze Telomerase Detection Kits and is used in the extraction step of the procedure.
Used to supplement the TRAPEZE Kit assay when larger tissue samples necessitate additional 1X CHAPS Lysis Buffer for efficient cell lysis. It extends the capabilities of the kits by enabling the analysis of larger tissue samples.
Application
TRAPeze™1X CHAPS Lysis Buffer has been used:
- to extract total proteins from HEK293 cells in quantitative telomerase activity assay (Q-TRAP)
- to homogenize mouse atrial tissues for western blots analysis
- in telomerase expression analysis of cell lines to resuspend the cells to determine protein concentration
Storage and Stability
Store at 4°C.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
TRAPEZE is a trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Yu-Feng Hu et al.
Biochimica et biophysica acta. Molecular basis of disease, 1867(5), 166088-166088 (2021-01-31)
Point mutation in alcohol dehydrogenase 2 (ALDH2), ALDH2*2 results in decreased catalytic enzyme activity and has been found to be associated with different human pathologies. Whether ALDH2*2 would induce cardiac remodeling and increase the attack of atrial fibrillation (AF) remains
Sigridur K Bodvarsdottir et al.
Mutation research, 729(1-2), 90-99 (2011-10-25)
In the present study the possible involvement of telomeres in chromosomal instability of breast tumors and cell lines from BRCA2 mutation carriers was examined. Breast tumors from BRCA2 mutation carriers showed significantly higher frequency of chromosome end-to-end fusions (CEFs) than
Manuel Bernabé-García et al.
Molecular oncology, 15(7), 1818-1834 (2021-03-14)
Telomerase reverse transcriptase (TERT) maintains telomere homeostasis, thus ensuring chromosome stability and cell proliferation. In addition, several telomere-independent functions of human TERT have been described. In this study, we report that TERT binds directly to the TCF binding elements located
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