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Synonym(s):
dextran sulfate buffer, hybridization accelerant, hybridization additive, northern blot hybridization component, southern blot hybridization component
UNSPSC Code:
41116012
eCl@ss:
32160405
NACRES:
NA.52
Recommended Products
packaging
pkg of 100 mL
Quality Level
manufacturer/tradename
Chemicon®
technique(s)
hybridization: suitable (nucleic acid)
shipped in
ambient
General description
Dextran Sulfate (MW >500,000) is used as an additive in probe hybridization solutions. Its presence accelerates the rate of hybridization by 10 fold. The addition of Dextran Sulfate favors the formation of probe networks, resulting in greater sensitivity during membrane-bound nucleic acid analysis. The product is supplied as a 50% (w/v) solution.
Application
Dextran Sulfate - 50% Solution
To prepare a solution containing 10% Dextran Sulfate, add 8 ml of hybridization ingredients to 2 ml of 50% Dextran Sulfate Solution.
See the "Hybrisol Nomograph for Dextran Sulfate" link located under the "Protocols" tab at catalog number S4030 product specific page on www.chemicon.com for determining the proper concentration of dextran sulfate needed for various hybridization condition guidelines.
To prepare a solution containing 10% Dextran Sulfate, add 8 ml of hybridization ingredients to 2 ml of 50% Dextran Sulfate Solution.
See the "Hybrisol Nomograph for Dextran Sulfate" link located under the "Protocols" tab at catalog number S4030 product specific page on www.chemicon.com for determining the proper concentration of dextran sulfate needed for various hybridization condition guidelines.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
Certificates of Analysis (COA)
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