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RSHMAG-69K

Millipore

MILLIPLEX® Rat Stress Hormone Magnetic Bead Panel - Endocrine Multiplex Assay

This MILLIPLEX MAP Rat Stress Hormone Magnetic Bead Panel is an Endocrine multiplex assay. The analytes available for this multiplex kit are: ACTH, Corticosterone, Melatonin.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

200

species reactivity

rat

manufacturer/tradename

Milliplex®

assay range

accuracy: 61%
(Corticosterone)
accuracy: 83%
(ACTH)
sensitivity: 0.9 pg/mL
(ACTH)
sensitivity: 1.52 pg/mL
(Melatonin)
sensitivity: 2914 pg/mL
(Corticosterone)
standard curve range: 0.55-400 pg/mL
(Melatonin)
standard curve range: 1.4-1,000 pg/mL
(ACTH)
standard curve range: 686-500,000 pg/mL
(Corticosterone)
inter-assay cv: <15%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Related Categories

General description

Stress is defined as the disruption of homeostasis through physical and/or psychological stimuli orstressors. Physiologically the neuroendocrine system, which includes the hypothalamus, pituitary and adrenal glands, plays a critical role in response to stress. Activated by corticotropin-releasing hormone (CRH) and arginine-vasopressin (AV), the pituitary gland releases ACTH which results in the secretion of cortisol / corticosterone and other glucocorticoids from the adrenal glands. These corticoids involve the whole body in the stress response and are involved in the termination of the stress response by a negative feedback loop to the hypothalamus. Corticosterone and melatonin are important in the regulation of the sleep-wake cycle.

MILLIPLEX® Rat Stress Hormone Panel is a 3-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, tissue/cell extracts or culture media samples: ACTH, corticosterone and/or melatonin. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Endocrine

Specificity

Cross Reactivty
This assay display insignificant cross-reactivity within the panel

Application

  • Analytes: ACTH, Corticosterone, Melatonin
  • Recommended Sample Type: Rat serum, plasma, cell/tissue supernatants and lysates
  • NOTE: For optimal assay performance of turbid serum and plasma samples an extraction protocol is provided.
  • Recommended Sample Dilution: 25μL per well is used for the following: undiluted extracted serum or plasma; 1:4 non-extracted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Endocrine
  • Research Subcategory: Metabolism

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Regulatory Information

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Certificates of Analysis (COA)

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Brooks A Gross et al.
Journal of neuroscience methods, 251, 83-91 (2015-05-28)
Sleep deprivation via gentle handling is time-consuming and personnel-intensive. We present here an automated sleep deprivation system via air puffs. Implanted EMG and EEG electrodes were used to assess sleep/waking states in six male Sprague-Dawley rats. Blood samples were collected
Chris P Jew et al.
PloS one, 8(8), e70415-e70415 (2013-08-14)
The group I metabotropic glutamate receptor 5 (mGluR5) has been implicated in the pathology of various neurological disorders including schizophrenia, ADHD, and autism. mGluR5-dependent synaptic plasticity has been described at a variety of neural connections and its signaling has been
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Although dietary iron is a determinant of iron status in animals, body fat mass has been reported to have an inverse association with iron status in human studies. The goal of this study was to determine the relationship between Fe
Milene L Brownlow et al.
Frontiers in molecular neuroscience, 10, 129-129 (2017-05-31)
Nutritional ketosis may enhance cerebral energy metabolism and has received increased interest as a way to improve or preserve performance and resilience. Most studies to date have focused on metabolic or neurological disorders while anecdotal evidence suggests that ketosis may
Viviane S Hambrecht-Wiedbusch et al.
Sleep, 40(9) (2017-09-22)
Sleep and pain are reciprocally related, but the precise mechanisms underlying this relationship are poorly understood. This study used a rat model of surgical pain to examine the effect of previous sleep loss on postoperative pain and tested the hypothesis
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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service