RMYOMAG-88K
MILLIPLEX® Rat Myokine Magnetic Bead Panel
Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enable the simultaneous analysis of multiple myokine and cytokine biomarkers in rat serum and plasma samples.
Sign Into View Organizational & Contract Pricing
All Photos(3)
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47
Quality Level
species reactivity
rat
manufacturer/tradename
Milliplex®
assay range
accuracy: 82%
(FGF21)
accuracy: 83%
(IL-15)
accuracy: 85%
(Fractalkine (FKN))
accuracy: 85%
(Irisin)
accuracy: 88%
(IL-6)
accuracy: 91%
(FSTL1)
accuracy: 94%
(LIF)
inter-assay cv: <20%
intra-assay cv: <10%
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
ambient
Related Categories
General description
Skeletal muscle is actively involved in the synthesis and secretion of many proteins collectively termed “myokines.” Muscle contraction during physical activity is an important activator for the release of myokines, which act in an autocrine and/or paracrine manner to regulate skeletal muscle growth. Myokines also act as endocrine hormones which mediate inter-organ crosstalk. Myokine theory provides molecular mechanistic explanations for exercise-induced metabolic changes in liver and adipose tissue as well as for profound changes in immune and neuron systems. Various rat disease models are now serving as exceptional research tools for myokine study.
MILLIPLEX® Rat Myokine Panel is a 12-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, and tissue culture samples: Brain-derived Neurotrophic Factor (BDNF), Erythropoeitin (EPO), Fibroblast Growth Factor 21 (FGF21), Follistatin-Like Protein 1 (FSTL-1), Fractalkine (FKN / CX3CL1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Myostatin (MSTN / GDF8), Osteocrin/Musclin (OSTN), Osteonectin (SPARC). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Cytokines/Chemokines
MILLIPLEX® Rat Myokine Panel is a 12-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, and tissue culture samples: Brain-derived Neurotrophic Factor (BDNF), Erythropoeitin (EPO), Fibroblast Growth Factor 21 (FGF21), Follistatin-Like Protein 1 (FSTL-1), Fractalkine (FKN / CX3CL1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Myostatin (MSTN / GDF8), Osteocrin/Musclin (OSTN), Osteonectin (SPARC). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Cytokines/Chemokines
Specificity
Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Application
- Analytes: Brain-Derived Neurotrophic Factor (BDNF), Erythropoietin (EPO), Fibroblast Growth Factor 21 (FGF-21), Follistatin-like Protein 1 (FSTL1), Fractalkine (FKN/CX3CL1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Growth/Differentiation Factor 8 (GDF8/Myostatin), Osteocrin (OSTN/Musclin), Secreted Protein Acid and Rich in Cysteine (Osteonectin/SPARC)
- Recommended Sample Type: Rat serum, plasma, cell/tissue culture supernatants and lysates
- Recommended Sample Dilution: 25μL per well of 1:2 diluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
- Assay Run Time: Overnight (16-18 hours) at 2-8°C
- Research Category: Metabolism, Endocrine
- Research Subcategory: Obesity, Metabolic Disorders Inflammation, Muscle Physiology
Features and Benefits
Design your multiplex kit by choosing available analytes within this panel.
Packaging
96 well plate
Storage and Stability
Recommended storage for kit components is 2 - 8°C.
Other Notes
Standard curve range: See protocol
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Target Organs
Respiratory Tract
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service