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RMHMAG-84K

Millipore

MILLIPLEX® Rat Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay

The analytes available for this multiplex kit are: Amylin (Active), C-Peptide 2, Ghrelin (Active), GIP (Total), GLP-1 (Active), Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY, TNF-α.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

200

species reactivity

rat

manufacturer/tradename

Milliplex®

assay range

accuracy: 103%
(Amylin (Active))
accuracy: 96%
(GLP-1 (Active))
accuracy: 96%
(IL-6)
accuracy: 96%
(MCP-1)
accuracy: 98%
(TNFα)
accuracy: 99%
(C-Peptide 2)
accuracy: 99%
(Insulin)
sensitivity: 1-62 pg/mL
standard curve range: 14-10,000 pg/mL
(Glucagon & TNFα)
standard curve range: 2.7-2,000 pg/mL
(GIP (Total))
standard curve range: 41-30,000 pg/mL
(Amylin (Active) & GLP-1 (Active))
standard curve range: 69-50,000 pg/mL
(C-Peptide 2, IL-6, Insulin, Leptin & MCP-1)
standard curve range: 7-5,000 pg/mL
(Ghrelin (Active), PP & PYY (Total))

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

Metabolic syndrome is a cluster of conditions, including increased blood pressure, elevated insulin levels, abnormal cholesterol levels and excess body fat around the waist. Key features of metabolic syndrome include insulin resistance, glucose intolerance, hypertension, dyslipidemia, and central obesity.

MILLIPLEX® Rat Metabolic Panel is a 13-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysate and culture supernatant samples and serum or plasma samples: Amylin (active), C-peptide 2, Active Ghrelin, GIP, GLP-1, Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Specificity

Cross Reactivty
No significant cross-reactivity with other hormones tested.

Application

  • Analytes: Amylin (Active), C-Peptide 2, Ghrelin (Active), GIP (Total), GLP-1 (Active), Glucagon, IL-6, Insulin, Leptin, MCP-1 (CCL2), Pancreatic Polypeptide (PP), PYY (Total), TNFα
  • Recommended Sample Type: Rat serum, plasma, cell/tissue culture supernatants and lysates.
  • NOTE: For specific analytes protease inhibitors should be added upon blood collection: DPPIV for GLP-1 (Active); Protease Inhibitor Cocktail for Amylin (Active); Aprotinin for Glucagon; Serine Protease Inhibitor for Ghrelin (Active)
  • Recommended Sample Dilution: 25 μL per well of neat serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
  • Assay Run Time: Overnight (18-20 hours) at 2-8°C
  • Research Category: Metabolism
  • Research Subcategory: Metabolic Disorders, Obesity, Endocrine

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

Supplementary Hazards

EUH032

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Regulatory Information

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Starr Villavasso et al.
Nutrients, 11(11) (2019-11-14)
We have previously shown that 6 weeks of intermittent high-fat diet (Int-HFD) pre-exposure significantly reduced alcohol drinking in rats, providing preliminary evidence of the effectiveness of a dietary intervention in reducing alcohol intake. However, the functional framework and underlying neurobiological
Jean-Baptiste Cavin et al.
Gastroenterology, 150(2), 454-464 (2015-10-21)
Bariatric procedures, such as Roux-en-Y gastric bypass (RYGB) or vertical sleeve gastrectomy (VSG), are the most effective approaches to resolve type 2 diabetes in obese individuals. Alimentary glucose absorption and intestinal disposal of blood glucose have not been directly compared
Sunil Sirohi et al.
Obesity (Silver Spring, Md.), 25(7), 1228-1236 (2017-05-14)
Roux-en-Y gastric bypass (RYGB) surgery reduces appetite and stimulates new onset alcohol misuse; however, the genesis of these behavioral changes is unclear. This study is hypothesized that new onset alcohol intake is a behavioral adaptation that occurs secondary to reduced
Alexander A Moghadam et al.
Experimental biology and medicine (Maywood, N.J.), 242(18), 1786-1794 (2017-12-02)
Alterations in gut hormone signaling are a likely contributing factor to the metabolic disturbances associated with overweight/obesity as they coordinate the timing of feeding behavior, absorption, and utilization of nutrients. These hormones are released in response to food intake, or
Laelie A Snook et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 309(3), R295-R303 (2015-06-05)
Several gastrointestinal proteins have been identified to have insulinotropic effects, including glucose-dependent insulinotropic polypeptide (GIP); however, the direct effects of incretins on skeletal muscle glucose transport remain largely unknown. Therefore, the purpose of the current study was to examine the
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Related Content

Multiplexing Metabolic Syndrome Biomarkers | MILLIPLEX® Assays

Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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