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RMHMAG-84K

MILLIPLEX® Rat Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay

The analytes available for this multiplex kit are: Amylin (Active), C-Peptide 2, Ghrelin (Active), GIP (Total), GLP-1 (Active), Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY, TNF-α.

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000

Key Documents

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Product Name

MILLIPLEX® Rat Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay, The analytes available for this multiplex kit are: Amylin (Active), C-Peptide 2, Ghrelin (Active), GIP (Total), GLP-1 (Active), Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY, TNF-α.

species reactivity

rat

manufacturer/tradename

Milliplex®

assay range

accuracy: 103%
(Amylin (Active))
accuracy: 96%
(GLP-1 (Active))
accuracy: 96%
(IL-6)
accuracy: 96%
(MCP-1)
accuracy: 98%
(TNFα)
accuracy: 99%
(C-Peptide 2)
accuracy: 99%
(Insulin)
sensitivity: 1-62 pg/mL
standard curve range: 14-10,000 pg/mL
(Glucagon & TNFα)
standard curve range: 2.7-2,000 pg/mL
(GIP (Total))
standard curve range: 41-30,000 pg/mL
(Amylin (Active) & GLP-1 (Active))
standard curve range: 69-50,000 pg/mL
(C-Peptide 2, IL-6, Insulin, Leptin & MCP-1)
standard curve range: 7-5,000 pg/mL
(Ghrelin (Active), PP & PYY (Total))

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Quality Level

200

Application

  • Analytes: Amylin (Active), C-Peptide 2, Ghrelin (Active), GIP (Total), GLP-1 (Active), Glucagon, IL-6, Insulin, Leptin, MCP-1 (CCL2), Pancreatic Polypeptide (PP), PYY (Total), TNFα
  • Recommended Sample Type: Rat serum, plasma, cell/tissue culture supernatants and lysates.
  • NOTE: For specific analytes protease inhibitors should be added upon blood collection: DPPIV for GLP-1 (Active); Protease Inhibitor Cocktail for Amylin (Active); Aprotinin for Glucagon; Serine Protease Inhibitor for Ghrelin (Active)
  • Recommended Sample Dilution: 25 μL per well of neat serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
  • Assay Run Time: Overnight (18-20 hours) at 2-8°C
  • Research Category: Metabolism
  • Research Subcategory: Metabolic Disorders, Obesity, Endocrine

Biochem/physiol Actions

Cross Reactivty
No significant cross-reactivity with other hormones tested.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Metabolic syndrome is a cluster of conditions, including increased blood pressure, elevated insulin levels, abnormal cholesterol levels and excess body fat around the waist. Key features of metabolic syndrome include insulin resistance, glucose intolerance, hypertension, dyslipidemia, and central obesity.

MILLIPLEX® Rat Metabolic Panel is a 13-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysate and culture supernatant samples and serum or plasma samples: Amylin (active), C-peptide 2, Active Ghrelin, GIP, GLP-1, Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

supp_hazards

EUH032

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Regulatory Information

新产品
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Maritza J Romero et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(7), 1895-1900 (2016-02-03)
Dyslipidemia associated with triglyceride-rich lipoproteins (TRLs) represents an important residual risk factor for cardiovascular and chronic kidney disease in patients with type 1 diabetes (T1D). Levels of growth hormone (GH) are elevated in T1D, which aggravates both hyperglycemia and dyslipidemia.
Carolina Soares Moura et al.
Food & nutrition research, 61(1), 1290740-1290740 (2017-03-23)
Background: Several physiologically beneficial effects of consuming a whey protein hydrolysate (WPH) have been attributed to the greater availability of bioactive peptides. Aims: The aim was to investigate the effect of four branched-chain amino acid- (BCAA-)containing dipeptides, present in WPH
Atanu Pal et al.
Diabetes, 64(6), 1941-1950 (2015-01-13)
The antidiabetes effects of Roux-en-Y gastric bypass (RYGB) are well-known, but the underlying mechanisms remain unclear. Isolating the proximal small intestine, and in particular its luminal glucose sensors, from the nutrient stream has been proposed as a critical change, but
Sunil Sirohi et al.
Obesity (Silver Spring, Md.), 25(7), 1228-1236 (2017-05-14)
Roux-en-Y gastric bypass (RYGB) surgery reduces appetite and stimulates new onset alcohol misuse; however, the genesis of these behavioral changes is unclear. This study is hypothesized that new onset alcohol intake is a behavioral adaptation that occurs secondary to reduced
Laelie A Snook et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 309(3), R295-R303 (2015-06-05)
Several gastrointestinal proteins have been identified to have insulinotropic effects, including glucose-dependent insulinotropic polypeptide (GIP); however, the direct effects of incretins on skeletal muscle glucose transport remain largely unknown. Therefore, the purpose of the current study was to examine the
View All Related Papers

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Quality Control Ranges: Milliplex Rat Metabolic Magnetic Bead Panel Catalog # RMHMAG-84K
96 well Template Single
Protocol- RMHMAG-84K
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