QIA117
Senescence Detection Kit
Synonym(s):
B-galactosidase senescence detection
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About This Item
UNSPSC Code:
12352207
Recommended Products
usage
sufficient for 250 tests
Quality Level
packaging
pkg of 1 12-well plate(s)
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
input
sample type intact cells
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
General description
Senescence is thought to be a tumor suppressive mechanism and an underlying cause of aging. It represents an arrested state in which the cells remain viable, but not stimulated to divide by serum or by passage in culture. Senescent cells display an increase in cell size, pH-dependent expression of β-galactosidase (β-Gal) activity, and altered patterns of gene expression.
Useful for the histochemical detection of senescent cells. The failure of cells to become senescent may lead to tumor progression. Higher levels of β-Galactosidase is considered as a good marker for senescent cells. The mechanism of this is believed to be due to the increased lysosomal content of senescent cells, resulting in an increase of the lysosomal enzyme β-galactosidase. Cells can be observed using microscope.
Components
Fixative Solution, X-Gal, Staining Solution, Staining Supplement, and a user protocol.
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Specifications
Assay Time: 30 min. plus overnight incubation
Principle
The Calbiochem® Senescence Detection Kit is useful for the histochemical detection of senescent cells. The failure of cells to become senescent may lead to tumor progression. The mechanism of this is believed to be the increased lysosomal content of senescent cells resulting in an increase of the lysosomal enzyme β-galactosidase.
Preparation Note
• PBS: Prepare a 1X PBS solution (not provided). Use at least 3 ml per well.• X-gal: Dissolve 20 mg X-gal in 1 ml of DMF (N-N-dimethylformamide, not provided) to prepare a 20X stock solution. Aliquot and freeze (-20°) the unused X-gal solution. This stock solution is stable for up to 1 month at -20°C (protect from light). Always store X-gal in a polypropylene or glass container. Do not use polystyrene.• Staining Solution Mix: While cells are incubating in Fixative Solution (step 2 below), prepare Staining Solution Mix in a polypropylene plastic tube by adding 470 µl of Staining Solution, 5 µl Staining Supplement, and 25 µl of 20 mg/ml X-gal in DMF.
Storage and Stability
Upon arrival store the entire contents of the kit at -20°C.
Other Notes
Kurz, D.J., et al. 2000. J. Cell Sci.113, 3613.
Dimri, G.P., et al. 1995. Proc. Natl. Acad. Sci. USA92, 9363.
Dimri, G.P., et al. 1995. Proc. Natl. Acad. Sci. USA92, 9363.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
Supplementary Hazards
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
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