PLHK07610
Regenerated Cellulose Membrane Filter, 100 kDa NMWCO
Ultracel®, filter diam. 76 mm
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Synonym(s):
Ultrafiltration Discs, 100 kDa NMW, Ultrafiltration membrane discs
UNSPSC Code:
41104903
eCl@ss:
32031602
NACRES:
NB.24
Recommended Products
material
regenerated cellulose membrane
Quality Level
manufacturer/tradename
Ultracel®
technique(s)
protein clean-up: suitable (desalting)
protein purification: suitable (concentration)
filter diam.
76 mm
filtration area
41.8 cm2
matrix
Ultracel®
pore size
100 kDa NMWCO
shipped in
ambient
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General description
Ultrafiltration membranes are generally composed of cellulose or polyethersulfone (PES). The material of choice will greatly depend on sample compatibility and the type of membrane will have a significant impact on performance. The molecular weight (MW) of the macrosolute that is to be retained determines the membrane nominal molecular weight cut-off (NMWCO). The diafiltration technique utilizes ultrafiltration membranes, either in centrifugal or pressure-driven devices such as the Amicon® Stirred Cell for efficient buffer exchange. The ultrafiltration membranes are preferred over dialysis tubing as the membranes can be used for both sample concentration and buffer exchange. This minimizes sample transfers and reduces sample loss.
Application
Ultrafiltration Discs, 100 kDa NMW has been used:
- in Millipore stirred ultrafiltration cell (MSUC) for glucose/ gluconic acid (GA) conversion catalyzed by glucose oxidase (GO)
- with Amicon® stirred cell model 8200 for microbial extracellular vesicles (EVs) isolation of large volumes cultures
- in the synthesis of polydopamine (pDA)-modified iron oxide core-shell nanoparticles (IONPs)
Linkage
Replaces: 14442AM
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Ester Junko Tomotani et al.
Applied biochemistry and biotechnology, 121-124, 149-162 (2005-05-27)
Glucose oxidase (GO) (EC 1.1.3.4) was used as catalyst for oxidizing glucose into gluconic acid utilizing a 10-mL Bioengineering Enzyme Membrane Reactor or a 400-mL Millipore Stirred Ultrafiltration Cell (MSUC) coupled with a Millipore UF membrane (cutoff of 100 kDa)
Ruihua Li et al.
Chemosphere, 243, 125391-125391 (2019-11-30)
In this study, novel lignin-based flocculant (LBF) was prepared in recycling of papermaking sludge. And LBF combined with polyaluminum chloride (PAC) was used in the coagulation/ultrafiltration combined process. Effects of polymer types, pH and ultrafiltration time on the membrane fouling
Nimisha Singh et al.
Nanomaterials (Basel, Switzerland), 9(2) (2019-01-27)
Polydopamine (pDA)-modified iron oxide core-shell nanoparticles (IONPs) are developed and designed as nanovectors of drugs. Reactive quinone of pDA enhances the binding efficiency of various biomolecules for targeted delivery. Glutathione disulfide (GSSG), an abundant thiol species in the cytoplasm, was
Carolina Coelho et al.
Bio-protocol, 10(2), e3502-e3502 (2020-01-20)
Extracellular vesicles (EVs) are produced by all domains of life including Bacteria, Archaea and Eukarya. EVs are critical for cellular physiology and contain varied cargo: virulence factors, cell wall remodeling enzymes, extracellular matrix components and even nucleic acids and metabolites.
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