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PICM03050

Millipore

Millicell® Standing Cell Culture Inserts

pore size 0.4 μm, diam. 30 mm, transparent PTFE membrane, hydrophilic, H 13 mm, size 6 wells, sterile

Synonym(s):

Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm, Millicell-CM, cell culture inserts, permeable culture insert, plate inserts, tissue culture insert, tissue culture plate

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About This Item

UNSPSC Code:
41104923
eCl@ss:
32011202
NACRES:
NB.22

material

polystyrene housing
transparent PTFE membrane

Quality Level

400

sterility

ethylene oxide treated
sterile

feature

hydrophilic

packaging

pack of 50 (individually blister packaged)

manufacturer/tradename

Millicell®

parameter

50 °C max. temp.

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable

H

13 mm

diam.

30 mm

filtration area

4.2 cm2

size

6 wells

surface area

4.2 cm2

working volume

1.5 mL

color

transparent membrane, when wetted

matrix

Biopore™

pore size

0.4 μm

binding type

low binding surface

detection method

fluorometric

shipped in

ambient

General description

With Millicell® standing inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® standing inserts make it possible to study both sides of the cell monolayer.

Application

  • Cell Attachment
  • Cell Growth
  • Cell Differentiation
  • Immunocytochemistry

Features and Benefits

  • Promotes excellent cell growth and provides an exceptional opportunity for cell studies
  • Low protein-binding hydrophilic PTFE membrane is ideal for live cell viewing and immunofluorescent application

Legal Information

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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CB1 receptors and post-ischemic brain damage: Studies on the toxic and neuroprotective effects of cannabinoids in rat organotypic hippocampal slices.
Landucci, Elisa, et al.
Neuropharmacology, 60, 674-682 (2011)
Synapse elimination in olivo-cerebellar explants occurs during a critical period and leaves an indelible trace in Purkinje cells.
Letellier, Mathieu, et al.
Proceedings of the National Academy of Sciences of the USA, 106, 14102-14107 (2009)
Daniela Buonvicino et al.
British journal of pharmacology, 177(14), 3342-3356 (2020-03-22)
Drugs able to counteract progressive multiple sclerosis (MS) represent a largely unmet therapeutic need. Even though the pathogenesis of disease evolution is still obscure, accumulating evidence indicates that mitochondrial dysfunction plays a causative role in neurodegeneration and axonopathy in progressive
Viktoria Melcher et al.
Acta neuropathologica, 139(5), 913-936 (2019-12-19)
Atypical teratoid/rhabdoid tumors (ATRT) are known for their heterogeneity concerning pathophysiology and outcome. However, predictive factors within distinct subgroups still need to be uncovered. Using multiplex immunofluorescent staining and single-cell RNA sequencing we unraveled distinct compositions of the immunological tumor
Preparation of organotypic hippocampal slice cultures: interface method.
De Simoni, Anna and Yu, Lily M Y
Nature Protocols, 1, 1439-1445 (2006)
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Protocols

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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