PF048
Heregulin, Human, Recombinant, E. coli
Synonym(s):
Neu Differentiation Marker, NDF
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About This Item
UNSPSC Code:
12352202
Assay
≥97% (SDS-PAGE)
Quality Level
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
wet ice
storage temp.
−20°C
General description
Recombinant, human heregulin expressed in E. coli. Heregulins have been shown to be mitogenic for Schwann cells and various epithelial cells. It is recommended that the protein be titrated for optimal results in individual systems.
Recombinant, human heregulin expressed in E. coli. Human recombinant heregulin whose DNA sequence encoding the EGF domain of heregulin α (amino acids 177-241) expressed in and purified from E. coli. The expressed protein consists of 65 amino acids with a predicted molecular weight of approximately 7 kDa.
Application
Proliferation Studies
Biochem/physiol Actions
EC₅₀ ~20-40 ng/ml as determined by a cell proliferation assay using breast carcinoma cell line SK-BR-3 or in a serum-free cell proliferation assay using the human cell line MCF7.
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from PBS, 50 µg BSA/µg cytokine.
Reconstitution
Store lyophilized protein dessicated at -20°C. Lyophilized product should be reconstituted in sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of no less than 50 µg/ml. Following initial thaw, aliquot and freeze (-20°C).
Other Notes
It is recommended that the protein be titrated for optimal results in individual systems.
Riese, D.J., et al. 1996. J. Biol. Chem.271, 20047.
Holmes, W.E., et al. 1992. Science256, 1205.
Karey, K.P., et al. 1988. Cancer Res.48, 4083.
Holmes, W.E., et al. 1992. Science256, 1205.
Karey, K.P., et al. 1988. Cancer Res.48, 4083.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
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Maximilian Haertinger et al.
Cells, 9(1) (2020-01-16)
Recent studies showed a beneficial effect of adipose stem cell-derived extracellular vesicles (ADSC-EVs) on sciatic nerve repair, presumably through Schwann cell (SC) modulation. However, it has not yet been elucidated whether ADSC-EVs exert this supportive effect on SCs by extracellular
Rachel R Besser et al.
Biomaterials science, 8(2), 591-606 (2019-12-21)
We report a water-soluble and non-toxic method to incorporate additional extracellular matrix proteins into gelatin hydrogels, while obviating the use of chemical crosslinkers such as glutaraldehyde. Gelatin hydrogels were fabricated using a range of gelatin concentrations (4%-10%) that corresponded to
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