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PCHT12H48

Millipore

Millicell® 12 well hanging cell culture inserts

pore size 0.4 μm, clear PET membrane, diam. 12 mm, Tissue Culture (TC)-treated surface, sterile

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Synonym(s):
permeable inserts, tissue culture inserts, transparent membrane insert, imaging insert
UNSPSC Code:
41122107
NACRES:
NB.13

material

clear PET membrane
polystyrene housing

Quality Level

200

sterility

γ-irradiated
sterile

feature

4x106 pores/cm2 (pore density)

packaging

pack of 48 ea (individually blister packaged)

manufacturer/tradename

Millicell®

parameter

max. temp. at 37 °C

technique(s)

cell attachment: suitable
cell differentiation: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
permeability assay: suitable

H

15.5 mm

diam.

12 mm

insert maximum volume

0.8 mL

insert working volume

0.4 mL

membrane thickness

9-16 μm

size

12 wells

surface area

1.1 cm2

color

clear membrane
 transparent membrane

pore size

0.4 μm pore size

binding type

Tissue Culture (TC)-treated surface

Related Categories

General description

Millicell® Cell Culture inserts with Polyethylene Terephthalate (PET) membrane are ideal for the growth and differentiation of various cell types. This individually sterile-packaged insert has an optically clear 0.4 μm membrane pore size and fits in most 12-well tissue culture plates.

Application

Barrier Assays
Cell Proliferation
Cell Surface Receptors
Co-Culture
Epithelial Cell Growth
Feeder Layers Fluorescent
Detection /Immunohistochemistry
In Vitro Fertilization
In Vitro Toxicology
Microbial Attachment
Phase Contrast Microscopy Polarized Protein Secretions
Polarized Uptake Transport/Permeability

Features and Benefits

  • Optically clear TC-treated PET membrane
  • Low pore density (4 million pores/cm2)
  • Compatible with most 12-well plates
  • Apical assist rim to prevent membrane puncture or disruption of cells
  • Easily access the basolateral side between the flanges

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

Barrier Formation and Permeability Assays using Millicell® Hanging Cell Culture Inserts

How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.

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