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biological source
rabbit
Quality Level
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG
shipped in
wet ice
storage temp.
2-8°C
General description
Anti-GluR1, rabbit polyclonal, recognizes the ~105-107 kDa GluR1 in D425 Med cells and hippocampal neurons. Also cross-reacts with a 50 kDa protein in rat brain. It is validated for WB and IF.
Purified rabbit polyclonal antibody. Recognizes the ~105-107 kDa GluR1 protein.
Recognizes the ~105-107 kDa GluR1 protein in D425 Med cells and hippocampal neurons. Also cross-reacts with a 50 kDa protein in rat brain extracts.
Immunogen
a synthetic peptide (RTSDSRDHTRVDWKR) corresponding to amino acids 271-285 of rat GluR1 (numbered from the signal peptide)
Application
Immunoblotting 1-5 µg/ml)
Immunofluorescence (10-20 µg/ml, see application references)
Immunofluorescence (10-20 µg/ml, see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Analysis Note
Positive Control
D425 Med cells, hippocampal neurons
D425 Med cells, hippocampal neurons
Other Notes
Also recognizes a smaller band at ~50 kDa using whole brain extracts. Immunofluorescence of dissociated cultured rat hippocampal neurons gave a punctate distribution, consistent with previous reports of GluR1 distribution. Antibody should be titrated for optimal results in individual systems.
Zuo, J. et al. 1997. Nature388, 769.
McHugh, T.J., et al. 1996. Cell87, 1339.
Tsien, J.Z., et al. 1996. Cell87, 1327.
Greenamyre, J.T. and Porter, R.H.P. 1994. Neurology44, S7.
Hollmann, M. and Heinemann, S. 1994. Ann. Rev. Neurosci.17, 31.
Bliss, T.V.P. and Collingridge, G.L. 1993. Nature361, 31.
Lomeli, H., et al. 1993. FEBS Lett.315, 318.
Molnar, E. et al. 1993. Neuroscience53, 307.
Advokat, C. and Pellegrin, A.I. 1992. Neurosci. Biobehav. Rev.16, 13.
Beal, M.F. 1992. FASEB J.6, 3338.
Rothman, S.M. 1992. Ann. NY Acad. Sci.648, 132.
McHugh, T.J., et al. 1996. Cell87, 1339.
Tsien, J.Z., et al. 1996. Cell87, 1327.
Greenamyre, J.T. and Porter, R.H.P. 1994. Neurology44, S7.
Hollmann, M. and Heinemann, S. 1994. Ann. Rev. Neurosci.17, 31.
Bliss, T.V.P. and Collingridge, G.L. 1993. Nature361, 31.
Lomeli, H., et al. 1993. FEBS Lett.315, 318.
Molnar, E. et al. 1993. Neuroscience53, 307.
Advokat, C. and Pellegrin, A.I. 1992. Neurosci. Biobehav. Rev.16, 13.
Beal, M.F. 1992. FASEB J.6, 3338.
Rothman, S.M. 1992. Ann. NY Acad. Sci.648, 132.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Regulatory Information
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Certificates of Analysis (COA)
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