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PC234L

Sigma-Aldrich

Anti-Vasopressin Rabbit pAb

lyophilized, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

contains

≤0.1% sodium azide as preservative

species reactivity

rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG

shipped in

ambient

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

rat ... Avp(24221)

General description

Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes vasopressin.
Recognizes vasopressin in rat hypothalamus.
This Anti-Vasopressin Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Vasopressin.

Immunogen

Rat
rat arginine vasopressin

Application


Frozen Sections (1:400-1:800, Cy3; 1:2000-1:4000, Bn-SA/HRP)
Immunofluorescence (1:200-1:400)

Warning

Toxicity: Standard Handling (A)

Physical form

Undiluted serum.

Reconstitution

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months. Avoid freeze/thaw cycles of solutions.

Analysis Note

Positive Control
Rat hypothalamus

Other Notes

Antibody specificity was examined in the rat paraventricular nucleus and supraoptic nucleus. Staining is completely eliminated by pretreatment of antibody with arginine vasopressin (10 µg/ml). Preabsorption of antibody with oxytocin (100 µg/ml) resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
Sudhof, T.C. 1995. Nature375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl.56, 154.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yarely C Hoffiz et al.
Scientific reports, 11(1), 2335-2335 (2021-01-29)
Long-standing clinical findings report a dramatic surge of vasopressin in umbilical cord blood of the human neonate, but the neural underpinnings and function(s) of this phenomenon remain obscure. We studied neural activation in perinatal mice and rats, and found that
Leqiang Sun et al.
Molecular neurodegeneration, 14(1), 8-8 (2019-02-10)
Neurotropic virus-based tracers have been extensively applied in mapping and manipulation of neural circuits. However, their neurotropic and neurotoxic properties remain to be fully characterized. Through neural circuit tracing, we systematically compared the neurotropism discrepancy among different multi-trans-synaptic and mono-synaptic
Zita Liutkevičiūtė et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, fj201800443-fj201800443 (2018-06-26)
Ants are emerging model systems to study cellular signaling because distinct castes possess different physiologic phenotypes within the same colony. Here we studied the functionality of inotocin signaling, an insect ortholog of mammalian oxytocin (OT), which was recently discovered in
V Tobin et al.
Journal of neuroendocrinology, 24(4), 629-641 (2011-10-13)
In magnocellular neurones of the supraoptic nucleus (SON), the neuropeptides vasopressin and oxytocin are synthesised and packaged into large dense-cored vesicles (LDCVs). These vesicles undergo regulated exocytosis from nerve terminals in the posterior pituitary gland and from somata/dendrites in the
Douglas W Wacker et al.
The Journal of physiology, 588(Pt 23), 4705-4717 (2010-10-06)
The anterior olfactory nucleus (AON), a component of the main olfactory system, is a cortical region that processes olfactory information and acts as a relay between the main olfactory bulbs and higher brain regions such as the piriform cortex. Utilizing

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