PC177L
Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb
lyophilized, Calbiochem®
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UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Slc6A4(15567)
General description
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the serotonin (5-HT) transporter protein.
Recognizes the serotonin (5-HT) transporter in hypothalamus raphe nuclei and spinal cord.
This Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Serotonin (5-HT) Transporter (602-622).
Immunogen
Rat
a synthetic peptide corresponding to amino acids 602-622 of rat 5-HT transporter
Application
Frozen Sections (1:10,000-1:15,000; see application references)
Immunoblotting (see comments)
Immunofluorescence (1:200-1:400)
Warning
Toxicity: Standard Handling (A)
Physical form
Undiluted serum.
Reconstitution
Reconstitute the lyophilized antibody with 100 µl of sterile distilled H₂O. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Further dilute with sterile PBS or Tris buffer to dilutions no higher than 1:10.
Analysis Note
Positive Control
Rat naphe nuclei, hypothalamus, cortex, or spinal cord
Rat naphe nuclei, hypothalamus, cortex, or spinal cord
Other Notes
Antibody specificity was examined in the rat cortex, hypothalamus, raphe nuclei, and spinal cord. Staining is completely eliminated by pretreatment of antibody with Serotonin (5-HT) Transporter Control Peptide (Cat. No. PP87) at a concentration of 5 µg/ml. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. The specificity of this antibody was also determined by immunoblotting analysis using rat brain extracts of the cortex, hypothalamus, midbrain, and hindbrain, which specifically detected a single band. Antibody should be titrated for optimal results in individual systems.
Zhou, F.C., et al. 1996. Mol. Brain Res.43, 267.
Blakely, R.D., et al. 1994. J. Exp. Biol.196, 263.
Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.
Blakely, R.D., et al. 1994. J. Exp. Biol.196, 263.
Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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