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antibody form
purified antibody
Quality Level
clone
polyclonal
species reactivity
goat
concentration
20 mg/mL
technique(s)
immunohistochemistry: suitable
General description
PAP soluble complexes are recommended when high sensitivity immunological detection of antigens or primary antibodies is required.
Whole antisera against IgG are suggested for bridging PAP to primary antibodies. Anti-whole IgG or anti-F(ab′)2 fragments of IgG will also bridge PAP to IgM primary antibodies, by virtue of common light chain recognition. Normal serums from the same host species as the bridging antibodies are suggested as blocking agents to minimize non-specific binding.
Whole antisera against IgG are suggested for bridging PAP to primary antibodies. Anti-whole IgG or anti-F(ab′)2 fragments of IgG will also bridge PAP to IgM primary antibodies, by virtue of common light chain recognition. Normal serums from the same host species as the bridging antibodies are suggested as blocking agents to minimize non-specific binding.
Specificity
SOLUBLE COMPLEX, Goat PAP
Physical form
Lyophilized. Buffer= 0.01M Sodium phosphate, 0.25 M NaCl containing 0.01% thimerosal, pH 7.6.
RECONSTITUTION: Reconstitute to proper volume with sterile distilled water. Let stand 1-2 hours at room temperature.
RECONSTITUTION: Reconstitute to proper volume with sterile distilled water. Let stand 1-2 hours at room temperature.
Storage and Stability
Maintain lyophilized material at 2-8°C for up to 12 months. After reconstitution, aliquot and freeze at -20°C in convenient undiluted aliquots for up to 6 months. WARNING: The use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
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