OP39
Anti-c-ErbB2/c-Neu (Ab-5) Mouse mAb (TA-1)
liquid, clone TA-1, Calbiochem®
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Synonym(s):
Anti-HER2, Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
TA-1, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
should not react with
mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... ERBB2(2064)
General description
Anti-c-ErbB2/c-Neu (Ab-5), mouse monoclonal, clone TA-, recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells. It is validated for FC, WB, IF, IP, and IHC on frozen sections.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing spleonocytes with SP2/0 mouse myeloma cells (see application references). Recognizes the c-ErbB2/c-neu protein.
Recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells.
Application
Flow Cytometry (5 µg/ml, see application references)
Frozen Sections (5 µg/ml)
Immunoblotting (see application references)
Immunofluorescence (5 µg/ml, see application references)
Immunoprecipitation (1 µg/sample, see application references)
Neutralization Studies (not recommended, see comments and application references)
Frozen Sections (5 µg/ml)
Immunoblotting (see application references)
Immunofluorescence (5 µg/ml, see application references)
Immunoprecipitation (1 µg/sample, see application references)
Neutralization Studies (not recommended, see comments and application references)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 50 mM PBS, 0.2% gelatin, pH 7.5.
Analysis Note
Positive Control
SK-BR-3 cells
SK-BR-3 cells
Other Notes
DiFiore, P.P., et al. 1987. Science237, 178.
Slamon, D.J., et al. 1987. Science235, 177.
Varley, J.M., et al. 1987. Oncogene1, 423.
Yamamoto, T., et al. 1986. Nature319, 230.
Blick, M., et al. 1984. Blood64, 1234.
Schwab, M., et al. 1984 Cold Spring Harbor Laboratory2, 215.
Bargmann, C.I., et al. 1968. Nature319, 226.
Slamon, D.J., et al. 1987. Science235, 177.
Varley, J.M., et al. 1987. Oncogene1, 423.
Yamamoto, T., et al. 1986. Nature319, 230.
Blick, M., et al. 1984. Blood64, 1234.
Schwab, M., et al. 1984 Cold Spring Harbor Laboratory2, 215.
Bargmann, C.I., et al. 1968. Nature319, 226.
Recognizes an extracellular epitope and does not cross react with human EGF-receptor. It will not inhibit in vivo protein tyrosine kinase activity. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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