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OP20

Sigma-Aldrich

Anti-c-Abl (Ab-3) Mouse mAb (24-21)

liquid, clone 24-21, Calbiochem®

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Synonym(s):
Anti-p150, Anti-Abl
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

24-21, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, human

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ABL1(25)

General description

Anti-c-Abl (Ab-3), mouse monoclonal, clone 24-21, recognizes the ~145 kDa human and ~150 kDa mouse c-Abl. It is validated for Western blotting, immunofluorescence, and immunoprecipitation.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with p.3U1 mouse myeloma cells (see application references). Recognizes the v-Abl, the ~145-150 kDa c-Abl, the ~210 kDa Bcr-Abl (CML), and the ~190 kDa Bcr-Abl (ALL) proteins.
Recognizes the ~145 kDa human c-Abl, the ~150 kDa mouse c-Abl, v-Abl, and the Bcr/Abl translocation products in ALL (~190 kDa) and CML (~210 kDa). Does not inhibit protein tyrosine kinase activity.
  • Antibody Target Gene Symbol: ABL1
  • Target Synonym: ABL, AI325092, bcr/abl, C-ABL, C-ABL 1B, CABL1, E430008G22Rik, JTK7, MGC117749, p145Abl, p150, v-abl
  • Entrez Gene Name: c-abl oncogene 1, receptor tyrosine kinase
  • Hu Entrez ID: 25 (Related Antibodies: PK1006PK1013OP19)
  • Mu Entrez ID: 11350
  • Rat Entrez ID: 311860

    • Immunogen

    a recombinant protein consisting of the carboxyl region of v-abl protein fused to TrpE

    Application

    Immunoblotting (1-5 µg/ml)

    Immunofluorescence (see application references)

    Immunoprecipitation (1-2 µg/sample)

    Neutralization Studies (not recommended)

    Packaging

    Please refer to vial label for lot-specific concentration.

    Warning

    Toxicity: Standard Handling (A)

    Physical form

    In 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.5.

    Analysis Note

    Positive Control
    K562 (human bcr/abl), HL-60 (normal abl), and ANN-1 (murine abl) cells

    Other Notes

    Does not inhibit protein tyrosine kinase activity. Detects human c-Abl (145 kDa), mouse c-Abl (150 kDa), v-Abl, and the Bcr/Abl translocation products in ALL (~210 kDa) and CML (~190 kDa). HL-60 and NIH3T3 cells contain normal, non-rearranged protein. Antibody should be titrated for optimal results in individual sample types.
    Wiedemann, L.M., et al. 1988. Blood71, 349.
    Stam, K., et al. 1985. N. Engl. J. Med.313, 1429.
    Konopka, J.B., et al. 1984. Cell37, 1035.
    Prywes, R., et al. 1983. Cell34, 569.
    de Klein, A., et al. 1982. Nature300, 765.
    Reynolds, F.H., Jr., et al. 1980. J. Virol.36, 374.
    Reynolds, F.H., Jr., et al. 1978. Proc. Natl. Acad. Sci. USA75, 3974.
    Witte, O.N., et al. 1978. Proc. Natl. Acad. Sci. USA75, 2488.
    Abelson, H.T. and Rabstein, L.S. 1970. Cancer Res.30, 2213.

    Legal Information

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

    WGK

    nwg

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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