NR07
Anti-Ryanodine Receptor Mouse mAb (C3-33)
liquid, clone C3-33, Calbiochem®
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UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
C3-33, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
fish, canine, rat, avian, amphibian, rabbit
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
rat ... Ryr2(689560)
General description
Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X 63 AG8.653 mouse myeloma cells (see application references). Supplied as protein G purified antibody. Recognizes the ~565 kDa ryanodine receptor.
Recognizes the ~565 kDa ryanodine receptor in rat brain hippocampus. A ~565 kDa doublet representing the α and β isoforms is observed in non-mammalian vertebrates.
This Anti-Ryanodine Receptor Mouse mAb (C3-33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation for the detection of Ryanodine Receptor.
Immunogen
Canine
canine cardiac ryanodine receptor (RYR)
Application
Frozen Sections (1 g/ml, see application references)
Immunoblotting (1 g/ml)
Immunoprecipitation (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Rat brain hippocampus
Rat brain hippocampus
Other Notes
A ~565 kDa doublet representing the α and β isoforms is observed in non-mammalian vertebrates. In frozen sections, Clone C3-33 strongly stains the myofiber consistent with the sarcoplasmic reticulum of the RyR. This antibody has been reported to work for immunoprecipitation; the concentration is assay-dependent. Antibody should be titrated for optimal results in individual systems.
Sitsapesan, R., et al. 1995. Trends Pharmacol. Sci.16, 386.
McPherson, P.S. and Campbell, K.P. 1993. J. Biol. Chem.268, 13765.
Meszaros, L.G., et al. 1993. Nature364, 76.
Lai, F.A., et al. 1992. Biochem. J.288, 553.
Lai, F.A., et al. 1989. Nature331, 315.
McPherson, P.S. and Campbell, K.P. 1993. J. Biol. Chem.268, 13765.
Meszaros, L.G., et al. 1993. Nature364, 76.
Lai, F.A., et al. 1992. Biochem. J.288, 553.
Lai, F.A., et al. 1989. Nature331, 315.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Carmen Tarifa et al.
JACC. Basic to translational science, 8(1), 1-15 (2023-02-14)
Analysis of the spatio-temporal distribution of calcium sparks showed a preferential increase in sparks near the sarcolemma in atrial myocytes from patients with atrial fibrillation (AF), linked to higher ryanodine receptor (RyR2) phosphorylation at s2808 and lower calsequestrin-2 levels. Mathematical
Ioannis Smyrnias et al.
Cell calcium, 47(3), 210-223 (2010-01-29)
Narrow, tubular, inward projections of the sarcolemma ('T-tubules') are an established feature of adult mammalian ventricular myocytes that enables them to generate the whole-cell Ca2+ transients and produce coordinated contraction. Loss of T-tubules can occur during ageing and under pathological
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