NE1023
Anti-Neurofilament H Non-Phosphorylated Mouse mAb (SMI-32)
liquid, clone SMI-32, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody product type
primary antibodies
clone
SMI-32, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
General description
This product has been discontinued.
We are offering Anti-Neurofilament H Non-Phosphorylated Mouse MAb (SMI-32) (Cat. No. 559844) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.
Recognizes the non-phosphorylated ~180 kDa-200 kDa neurofilament H protein in rat central nervous system (CNS) cytoskeletal preparations.
Mouse monoclonal antibody supplied as undiluted ascites. Recognizes the ~180-200 kDa non-phosphorylated neurofilament H protein.
Immunogen
Rat
homogenized hypothalami from Fischer 344 rat brain
Application
ELISA (1:1000)
Frozen Sections (1:1000, see comments)
Immunoblotting (1:1000, see comments)
Immunocytochemistry (1:1000, see comments)
Paraffin Sections (1:1000, heat pre-treatment required, see comments)
Warning
Toxicity: Standard Handling (A)
Physical form
Undiluted ascites.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Rat brain or central nervous system cytoskeletal preparations
Rat brain or central nervous system cytoskeletal preparations
Other Notes
Only recognizes non-phosphorylated neurofilament H. By immunocytochemistry this antibody stains neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems, but does not stain thin axons or other cells and tissues. Tissues and cultured cells can be fixed in a variety of paraformaldehyde- or formaldehye-containing fixatives, such as Bouin′s fixative. Antibody reactivity is poor in glutaraldehyde/paraformaldehyde-fixed samples. For staining formalin-fixed, paraffin sections it is recommended that de-paraffinized tissue be autoclaved in dH2O for 10 min or boiled in Tris-buffered saline, pH 9.0, in a microwave for 15 min. Trypsin pre-treatment abolishes antibody binding to the epitope. Post-fixation in cold methanol or methanol/hydrogen peroxide facilitates access of the antibody to the neurons in frozen sections or thick sections fixed in 4% paraformaldehyde and in cultured cells. By immunoblotting this antibody detects two bands, ~180 and ~200 kDa, which merge into a single NFH line on two-dimensional gels. Antibody should be titrated for optimal results in individual systems.
Trapp, B.D., et al. 1998. N. Engl. J. Med.338, 278.
King, C.E., et al. 1997. Neuroreport.8, 1663.
Campbell, M.J., et al. 1991. Brain Res.539, 133.
Campbell, M.J., et al. 1989. J. Comp. Neurol.282, 191.
Sternberger, L.A., et al. 1983. Proc. Natl. Acad. Sci. USA80, 6126.
King, C.E., et al. 1997. Neuroreport.8, 1663.
Campbell, M.J., et al. 1991. Brain Res.539, 133.
Campbell, M.J., et al. 1989. J. Comp. Neurol.282, 191.
Sternberger, L.A., et al. 1983. Proc. Natl. Acad. Sci. USA80, 6126.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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