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MHA000P2N

Millipore

Wallerstein Nutrient Broth

ready-to-use, ampule of 2 mL, for bacteria, for molds, for yeasts, suitable for microbiology

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Synonym(s):
WL Nutrient broth, WLNB, Wallerstein Laboratory Nutrient Broth
UNSPSC Code:
41106225
eCl@ss:
32160801
NACRES:
NA.74

form

liquid

Quality Level

100

shelf life

12 mo.

feature

ready-to-use

packaging

ampule of 2 mL
pack of 50 tests

color

slightly opalescent green-blue

input

sample type bottled water
sample type beer
sample type sport drink
sample type cider
sample type food(s)
sample type beverage(s)
sample type wine
sample type cosmetics
sample type soft drink
sample type beverage(s)

pH

5.5±0.2 (25.0 °C)

application(s)

environmental
food and beverages
industrial qc
microbiology

compatibility

for use with MF-method
for use with Microfil®
for use with MicropreSure®
for use with Milliflex®

shipped in

wet ice

storage temp.

2-8°C

suitability

bacteria
molds
yeasts

Related Categories

General description

Wallerstein Nutrient Broth is recommended for the isolation and cultivation of yeasts, molds and bacteria that grow in a low pH environment mainly in the beverage industry. Yeast extract in the medium is a source of trace elements, vitamins and amino acids. Peptone provides nitrogen, amino acids and carbon. Dextrose is a source of carbohydrates. Monopotassium phosphate buffers the media. Potassium chloride, calcium chloride, and ferric chloride are essential ions to help to maintain osmotic balance. Magnesium sulfate and manganese sulfate are sources of divalent cations. Bromocresol green is the pH indicator. The WL Nutrient medium plate incubated under aerobic conditions enables the growth of mainly yeast colonies, acetic acid bacteria, flavobacterium, proteus and thermophilic bacteria and under anaerobic condition enables the growth of lactic acid bacteria and Pediococcus. Bacterial colonies appear as non-pigmented to white, with a smooth or rough texture. Yeast appears as large creamy to white colonies.

Application

Wallerstein Nutrient Broth is used in the examination of materials encountered in brewing and for industrial fermentations containing mixed flora of yeast and bacteria. It is used in the beverage industry to detect bacteria, yeast, and mold in wines, beer and other fermentation products.

Features and Benefits

  • Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment, and trained personnel are often in short supply.
  • They are available in convenient, single-test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.

Specifications

Incubation Temperature: 35 °C ± 2 for bacteria; 30 °C ± 2 for yeasts and molds
Incubation Time: 48–72 hrs

Analysis Note

Bacterial colonies can appear non-pigmented to white, with a smooth or rough texture. Yeast appear as creamy, white larger colonies
QC organisms
L. fermentum (ATCC 9338)

S. cerevisiae (ATCC 9763)
Target Microorganisms: Lactic acid bacteria, yeast and mold

Legal Information

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Media for Isolation-Identification-Cultivation-Maintenance of Medical Bacteria
MacFaddin JF
Williams and Wilkins, 12, 353-353 (1985)
Paper read at American Society of Brewing Chemists Meeting
Green SR and Gray PP
Wallerstein Laboratories Communications, 12, 353-353 (1950)

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