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MHA000P2F

Millipore

m-FC Broth with Rosolic Acid

ready-to-use, ampule of 2 mL, suitable for microbiology, for fecal coliforms

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Synonym(s):
m-FC Broth
UNSPSC Code:
41106225
eCl@ss:
32160801
NACRES:
NA.74

form

liquid

Quality Level

100

shelf life

12 mo.

feature

ready-to-use

packaging

ampule of 2 mL
pack of 50 tests

color

deep redslightly opalescent

input

sample type dairy
sample type wine
sample type bottled water
sample type beer
sample type food(s)
sample type soft drink
sample type beverage(s)
sample type cider

pH

7.4±0.2 (25.0 °C)

application(s)

environmental
microbiology

compatibility

for use with EZ-Fit® Filtration Manifold
for use with MF-method
for use with MicropreSure®
for use with Milliflex®

shipped in

wet ice

storage temp.

2-8°C

suitability

fecal coliforms

Related Categories

General description

m-FC Broth with Rosolic Acid is used for the detection and enumeration of fecal coliforms in water and other liquid samples by membrane filtration technique. This medium is based on the property that fecal coliforms grow at 44-45°C. Enzymatic digestion of animal tissue provides nitrogen, carbon, and minerals in M-FC Broth with Rosolic Acid. Yeast Extract is a source of vitamins and trace elements. Sodium Chloride maintains the osmotic balance. Lactose serves as a carbohydrate source. Bile Salts inhibit the growth of Gram-positive bacteria. Rosolic Acid is the differential indicator system. Coliforms form blue colonies, whereas non-coliforms form gray-colored colonies.

Application

m-FC Broth with Rosolic Acid is suitable for testing waste and effluent waters for fecal coliforms.

Features and Benefits

  • Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment, and trained personnel are often in short supply.
  • They are available in convenient, single-test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.

Specifications

Incubation Temperature: 44.5 °C ± 0.2
Incubation Time: 24 hrs ± 2
Standard Methods for the Examination of Water and Wastewater

Analysis Note

Fecal coliforms appear blue, other colonies appear gray to cream colored. In some rare cases, a membrane may have confirmed fecal coliforms that are pink in color. This may be due to insufficient medium on the petri pad, excess moisture loss from Petri dish during incubation, or delayed observation of the colonies.
QC organisms
E. coli (ATCC 25922)

Mixed culture (E. coli, P. aeruginosa and P. vulgaris)
Target Microorganisms: Fecal coliforms

Legal Information

EZ-FIT is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mackie & McCartney practical medical microbiology
Mackie TJ
J. Amer. Water Works Assoc., 57(2), 208-214 (1996)
Fecal-coliform-organism medium for the membrane filter technique
Geldreich EE et al.
J. Amer. Water Works Assoc., 57(2), 208-214 (1965)

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