MABT913
Anti-Cytokeratin 19 Antibody, clone TROMA-3
clone TROMA-3, from rat
Synonym(s):
Keratin, type I cytoskeletal 19, CK-19, Keratin-19, K19
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
TROMA-3, monoclonal
species reactivity
mouse, human
packaging
antibody small pack of 25 μg
technique(s)
electron microscopy: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... KRT19(3880)
mouse ... Krt19(16669)
Related Categories
General description
Keratin, type I cytoskeletal 19 (UniProt: P19001; also known as Cytokeratin-19, CK-19, Keratin-19, K19) is encoded by the Krt19 (also known as Krt1-19) gene (Gene ID: 16669) in murine species. CK-19 belongs to the family of intermediate filament (IF) family, but differs from other IF proteins in lacking the C-terminal tail domain. About 20 different cytokeratin proteins have been reported in higher animals in varying combinations during various stages of development. CK-19 is normally expressed in the lining of the gastroenteropancreatic and hepatobiliary tracts. It is expressed throughout embryonic development with highest levels observed at 8.5 days post coitum. Its expression is shown to decrease by day 11.5 and then is increased again by 17.5 days post coitum. CK-19 is shown to be involved in the organization of myofibrils and together with keratin 8, it helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK-19 has been shown to be an independent prognostic factor for pancreatic neuroendocrine tumors, especially the insulin-negative tumors. TROMA 3 has been found to stain trophoblastoma TDM.1 cells, a subpopulation of the retinoic acid.-induced F9 and PCC3/ND1 cells, and in 5-azacytidine-treated 1246 cells.
Specificity
Clone TROMA-3 detects cytokeratin 19 in human and murine cells.
Immunogen
Purified intermediate filaments from DM1 (Trophoblatoma) cell line.
Application
Anti-Cytokeratin 19, clone TROMA-3, Cat. No. MABT913, is a rat monoclonal antibody that detects Keratin, type I cytoskeletal 19 and is tested for use in Electron Microscopy, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Cytokeratin 19 in mouse lung tissue.
Western Blotting Analysis: A representative lot detected Cytokeratin 19 in Western Blotting applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).
Electron Microscopy Analysis: A representative lot detected Cytokeratin 19 in Electron Microscopy applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).
Immunofluorescence Analysis: A representative lot detected Cytokeratin 19 in Immunofluorescence applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).
Immunohistochemistry Analysis: A representative lot detected Cytokeratin 19 in Immunohistochemistry applications (Takase, H.M., et. al. (2013). Genes Dev. 27(2):169-81).
Immunoprecipitation Analysis: A representative lot detected Cytokeratin 19 in Immunoprecipitation applications (Ichinose, Y., et. al. (1990). Biochem Biophys Res Commun. 167(2):644-7).
Western Blotting Analysis: A representative lot detected Cytokeratin 19 in Western Blotting applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).
Electron Microscopy Analysis: A representative lot detected Cytokeratin 19 in Electron Microscopy applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).
Immunofluorescence Analysis: A representative lot detected Cytokeratin 19 in Immunofluorescence applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).
Immunohistochemistry Analysis: A representative lot detected Cytokeratin 19 in Immunohistochemistry applications (Takase, H.M., et. al. (2013). Genes Dev. 27(2):169-81).
Immunoprecipitation Analysis: A representative lot detected Cytokeratin 19 in Immunoprecipitation applications (Ichinose, Y., et. al. (1990). Biochem Biophys Res Commun. 167(2):644-7).
Quality
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cytokeratin 19 in 10 µg of MCF-7 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cytokeratin 19 in 10 µg of MCF-7 cell lysate.
Target description
~44 kDa observed; 44.54 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
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