MABT880
Anti-SUN2 Antibody, clone 3.1E
clone 3.1E, from mouse
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Synonym(s):
SUN domain-containing protein 2, Protein unc-84 homolog B, Rab5-interacting protein, Rab5IP, Sad1/unc-84 protein-like 2
UNSPSC Code:
12352203
eCl@ss:
32160702
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biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
3.1E, monoclonal
species reactivity
human, rat, mouse
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... SUN2(25777)
General description
SUN domain-containing protein 2 (UniProt Q9UH99; also known as Protein unc-84 homolog B, Rab5-interacting protein, Rab5IP, Sad1/unc-84 protein-like 2) is encoded by the SUN2 (also known as FRIGG, KIAA0668, RAB5IP, UNC84B) gene (Gene ID 25777) in human. It is a single-pass nuclear envelope transmembrane protein that is highly expressed in heart, lung, and muscle. It has a predicted transmembrane domain and a C-terminal region with similarity to the S. pombe spindle pole body protein Sad1. It may also facilitate a nuclear-centrosomal interaction required for nuclear migration and anchorage. It anchors chromosome movement in the prophase of meiosis and is required for telomere attachment to nuclear envelope and gametogenesis. SUN2 protein may also function on endocytic vesicles as a receptor for RAB5-GDP and participate in the activation of RAB5. Slight overexpression of SUN2 protein is seen in heart tissues form patients with congenital heart defects.
Specificity
Target specificity of clone 3.1E was verified by immunocytochemistry and Western blotting analyses using fibroblasts and heart tissue samples from Sun2-knockout mice.
Immunogen
GST-tagged recombinant human SUN2 N-terminal LMNA-binding region fragment.
Application
Research Category
Cell Structure
Cell Structure
This mouse monoclonal Anti-SUN2 Antibody, clone 3.1E, Cat. No. MABT880, is validated for use in Immunocytochemistry and Western Blotting for the detection of SUN2.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected SUN2 in 10 µg of HepG2 cell lysate.
Immunocytochemistry Analysis: A representative lot immunostained nucleus of fibroblasts from Sun2+/-, but not Sun2-/- mice (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Western Blotting Analysis: A representative lot detected SUN2 in heart tissue lysate from Sun2+/-, but not Sun2-/- mice (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Immunofluorescence Analysis (IF): A representative lot detected mouse testis tissue (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Immunofluorescence Analysis (IF): A representative lot tested on different mouse tissues (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Immunofluorescence Analysis (IF): A representative lot detected immortalized fibroblasts derived from SUN2 +/+, SUN2 -/- , Sun1 -/- double knockout Sun2 -/- Sun1 -/- mouse pups (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Immunocytochemistry Analysis: A representative lot immunostained nucleus of fibroblasts from Sun2+/-, but not Sun2-/- mice (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Western Blotting Analysis: A representative lot detected SUN2 in heart tissue lysate from Sun2+/-, but not Sun2-/- mice (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Immunofluorescence Analysis (IF): A representative lot detected mouse testis tissue (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Immunofluorescence Analysis (IF): A representative lot tested on different mouse tissues (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Immunofluorescence Analysis (IF): A representative lot detected immortalized fibroblasts derived from SUN2 +/+, SUN2 -/- , Sun1 -/- double knockout Sun2 -/- Sun1 -/- mouse pups (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected SUN2 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected SUN2 in 10 µg of HeLa cell lysate.
Target description
~80 kDa observed. 80.31/82.50/79.61 kDa (isoform 1/2/3) calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified.
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Fangfang Yang et al.
Frontiers in bioengineering and biotechnology, 8, 647-647 (2020-07-17)
Atherosclerotic plaque preferentially develops in arterial curvatures and branching regions, where endothelial cells constantly experience disturbed blood flow. By contrast, the straight arteries are generally protected from plaque formation due to exposure of endothelial cells to vaso-protective laminar blood flow.
Disulfide bond in SUN2 regulates dynamic remodeling of LINC complexes at the nuclear envelope.
Sharma, et al.
Life science alliance, 6 (2023)
Andres Ramirez-Martinez et al.
Nature communications, 12(1), 690-690 (2021-01-31)
Lamins and transmembrane proteins within the nuclear envelope regulate nuclear structure and chromatin organization. Nuclear envelope transmembrane protein 39 (Net39) is a muscle nuclear envelope protein whose functions in vivo have not been explored. We show that mice lacking Net39 succumb
Joana T Lima et al.
Life science alliance, 7(4) (2024-01-17)
Accurate centrosome separation and positioning during early mitosis relies on force-generating mechanisms regulated by a combination of extracellular, cytoplasmic, and nuclear cues. The identity of the nuclear cues involved in this process remains largely unknown. Here, we investigate how the
Yichi Zhang et al.
The Journal of clinical investigation, 133(13) (2023-07-03)
Mutations in genes encoding nuclear envelope proteins lead to diseases known as nuclear envelopathies, characterized by skeletal muscle and heart abnormalities, such as Emery-Dreifuss muscular dystrophy (EDMD). The tissue-specific role of the nuclear envelope in the etiology of these diseases
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