Anti-MYH-4 Antibody, clone 2G72F10

Myosin-4 (UniProt: Q5SX39; also known as Myosin heavy chain 2b, MyHC-2B, Myosin heavy chain 4) is encoded by the Myh4 gene (Gene ID: 17884) in murine species. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular sub-fragments (S1) and 1 rod-shaped sub-fragment (S2). In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and non-muscle cells. These isoforms show expression that is spatially and temporally regulated during development. Myosin-4 contains an N-terminal SH3-like domain (aa 33-82), a myosin motor domain (aa 86-782), and an IQ domain (aa 785-814). It also contains 2 actin binding regions (aa 659-681 and 761-775).

Clone 2G72F10 is a rat monoclonal antibody that detects Myosin-4 in mouse and rat muscles. It targets an epitope within 29 amino acids from the C-terminal half.

KLH-conjugated linear peptide corresponding to 29 amino acids from the C-terminal half of murine Myosin-4.

Anti-MYH-4, clone 2G72F10, Cat. No. MABT847, is a rat monoclonal antibody that detects Myosin-4 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected MYH-4 in Immunohistochemistry applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

Western Blotting Analysis: A representative lot detected MYH-4 in Western Blotting applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

Immunofluorescence Analysis: A representative lot detected MYH-4 in Immunofluorescence applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

Immunohistochemistry Analysis: A 1:25 dilution from a representative lot detected MYH-4 in mouse heart and mouse skeletal muscle tissues.

Research Category
Cell Structure

Evaluated by Western Blotting in mouse soleus muscle tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected MYH-4 in mouse soleus muscle tissue lysate.

~220 kDa observed; 222.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.