MABT411
Anti-Desmocollin-2 Antibody, clone 7G6
clone 7G6, from mouse
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Desmocollin-2, Cadherin family member 2, Desmocollin-3, Desmosomal glycoprotein II, Desmosomal glycoprotein III
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
7G6, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... DSC3(1825)
General description
Desmocollin-2, also known as Desmocollin-3 or Desmosomal glycoprotein II, or Desmosomal glycoprotein III, and encoded by the gene DSC2/CDHF2/DSC3, is a component of intercellular desmosome junctions. Desmocollin-2 is involved in the interaction of plaque proteins and intermediate filaments that mediate cell-cell adhesion and interacts with DSP, PKP2 and JUP proteins. Additionally, Desmocollin-2 is important for epidermal cell positioning and stratification because it mediates the differential adhesiveness between cells that express different isoforms. Desmocollin-2 is localized to the cell membrane at cell junctions and desmosomes. Desmocollin-2 is expressed in epithelia, myocardium and lymph nodes. Defects in Desmocollin-2 may be associated with Familial arrhythmogenic right ventricular dysplasia 11 (ARVD11) a congenital heart disease characterized by infiltration of adipose and fibrous tissue into the right ventricle and loss of myocardial cells, resulting in ventricular and supraventricular arrhythmias.
Immunogen
Epitope: This antibody recognizes the extracellular domain of Desmocollin-2.
MBP-tagged recombinant protein corresponding to the extracellular domain of human Desmocollin-2.
Application
Immuonoprecipitation Anlaysis: A representative lot from an independent laboratory detected Desmocollin-2 in A431 cells transfected with C-terminal deletions of plakoglobin retaining 707 amino acids (Wahl, J. K. 3rd, et al. (1996). J Cell Sci. 109(Pt 5):1143-1154.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Desmocollin-2 in Primary human Keratinocytes (Franke, WW., et al (2006). Eur. J. Cell Biol. 85(2); 69-82.)
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Desmocollin-2 in A431 cells (Wahl, J. K. 3rd. (2005). J Cell Biochem. 96(2):390-403.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Desmocollin-2 in Primary human Keratinocytes (Franke, WW., et al (2006). Eur. J. Cell Biol. 85(2); 69-82.)
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Desmocollin-2 in A431 cells (Wahl, J. K. 3rd. (2005). J Cell Biochem. 96(2):390-403.).
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
This Anti-Desmocollin-2 Antibody, clone 7G6 is validated for use in western blotting, IP, IHC & ICC for the detection of Desmocollin-2.
Quality
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Desmocollin-2 in 10 µg of A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Desmocollin-2 in 10 µg of A431 cell lysate.
Target description
~110 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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