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MABT334

Sigma-Aldrich

Anti-WASF2/WAVE-2 Antibody, clone ZZ36-12

clone ZZ36-12, from mouse, purified by affinity chromatography

Synonym(s):

WASF2, WAVE-2, Wiskott-Aldrich syndrome protein family member 2, WASP family protein member 2, Protein WAVE-2, Verprolin homology domain-containing protein 2

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CN¥234.05
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CN¥384.49
500 G
CN¥1,569.17

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Estimated to ship onMarch 03, 2025Details


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25 G
CN¥234.05
100 G
CN¥384.49
500 G
CN¥1,569.17

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

CN¥234.05


Estimated to ship onMarch 03, 2025Details


Request a Bulk Order

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ZZ36-12, monoclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

General description

Wiskott-Aldrich Syndrome Protein (WASP)-family Protein Member 2 (known as WASF2/WAVE-2) has a very wide distribution with strong expression in peripheral blood leukocytes. WASF2/WAVE-2 is involved in signaling RTKs and small GTPases. In general WAVE family of proteins have a general function for regulating the actin cytoskeleton in various tissues.

Immunogen

Epitope: C-terminus
His-tagged recombinant protein corresponding to the C-terminus of Human WASF2/WAVE-2.

Application

Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
This Anti-WASF2/WAVE-2 Antibody, clone ZZ36-12 is validated for use in Western Blotting and Immunocytochemistry for the detection of WASF2/WAVE-2.
Western Blotting Analysis: 1 µg/mL of a representative lot of this antibody detected WASF2/WAVE-2 in 10 µg of K562 cell lysate.

Western Blotting Analysis: A representative lot of this antibody detected WASF2/WAVE-2 in HeLa cell lysate +/- RNAi.

Immunocytochemistry Analysis: A representative lot of this antibody detected WASF2/WAVE-2 in HeLa cells.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected WASF2/WAVE-2 in 10 µg of HeLa cell lysate.

Target description

~65 kDa observed.
May run at ~65-80 kDa, which is higher than calculated molecular weight of 54 kDa, due to the proline rich residues in the sequence (Yan, C., et al. (2003) EMBO J. 22(14): 3602–3612).

Physical form

Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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