Skip to Content
Merck
CN
All Photos(3)

Documents

MABT329M

Sigma-Aldrich

Anti-Cytokeratin 8 Antibody, clone TROMA-1

clone TROMA-1, from rat

Synonym(s):

Keratin type II cytoskeletal 8, Cytokeratin endo A, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TROMA-1, monoclonal

species reactivity

human, mouse

packaging

antibody small pack of 25 μg

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... KRT8(3856)
mouse ... Krt8(16691)

General description

Keratin, type II cytoskeletal 8 (UniProt: P11679; also known as Cytokeratin endo A, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8) is encoded by the Krt8 (also known as Krt2-8) gene (Gene ID: 16691) in murine species. Cytokeratins belong to a diverse group of intermediate filaments that participate in differentiation and maintain the structural integrity of epithelial cells. Cytokeratins share a common structural organization: a central a-helical rod domain flanked by head and tail domains. An important unique feature of cytokeratins is their epithelial cell-type-specific expression. CK8 is a heterotetramer of two type I and two type II keratins. It is expressed in abundance in the epithelia of colon, bladder, ileum, and stomach. CK8 together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK8 is often used together with keratin 18 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. CK8 expression has been correlated with malignancy in leukoplakia and carcinomas of the head and neck and its expression has been observed in all non-small-cell lung cancers.

Specificity

Clone TROMA-1 detects cytokeratin-8 in human and murine cells.

Immunogen

Intermediate filaments extracted from TDM1 Trophoblastoma cells.

Application

Immunocytochemistry Analysis: A representative lot detected Cytokeratin 8 in Immunocytochemistry applications (O′Driscoll, C.M., et. al. (2013). Biochim Biophys Acta. 1833(3):460-7; Wang, X., et. al. (2004). J Cell Sci. 117(Pt 1):53-62).

Western Blotting Analysis: A representative lot detected Cytokeratin 8 in Western Blotting applications (Helenius, T.O., et. al. (2016). Cells. 5(3); O′Driscoll, C.M., et. al. (2013). Biochim Biophys Acta. 1833(3):460-7; Tao, G.Z., et. al. (2003). J Cell Sci. 116(Pt 22):4629-38; Maurer, J., et. al. (2008) PLoS One. 3(10):e3451).

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Cytokeratin 8 in HepG2 cells.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cytokeratin 8 in mouse lung tissue.

Immunoprecipitation Analysis: A representative lot detected Cytokeratin 8 in Immunoprecipitation applications (Brulet, P., et. al. (1982). Proc Natl Acad Sci USA. 79(7):2328-32).

Radioimmunoassay Analysis: A representative lot detected Cytokeratin 8 in Radioimmunoassy applications (Brulet, P., et. al. (1982). Proc Natl Acad Sci USA. 79(7):2328-32).

Immunofluorescence Analysis: A representative lot detected Cytokeratin 8 in Immunofluorescence applications (Helenius, T.O., et. al. (2016). Cells. 5(3); Tao, G.Z., et. al. (2003). J Cell Sci. 116(Pt 22):4629-38; Lin, Y.M., et. al. (2008). J Cell Sci. 121(Pt 14):2382-93; Maurer, J., et. al. (2008) PLoS One. 3(10):e3451; Nuotio-Antar, A.M., et. al. (2006). J Steroid Biochem Mol Biol. 99(2-3):93-9; Tamai, Y., et. al. (2000). J Cell Biol. 151(3):563-72).

Immunohistochemistry Analysis: A representative lot detected Cytokeratin 8 in Immunohistochemistry applications (Szeder, V., et. al. (2003). Dev Biol. 253(2):258-63; Tao, G.Z., et. al. (2003). J Cell Sci. 116(Pt 22):4629-38; Nuotio-Antar, A.M., et. al. (2006). J Steroid Biochem Mol Biol. 99(2-3):93-9; Tamai, Y., et. al. (2000). J Cell Biol. 151(3):563-72).
This rat monoclonal Anti-Cytokeratin 8, clone TROMA-1, Cat. No. MABT329, is a rat monoclonal antibody that detects cytokeratin-8 in human and murine cells & is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Radioimmunoassay, & WB.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 ug/mL of this antibody detected Cytokeratin 8 in 10 µg of HeLa cell lysate.

Target description

~54 kDa observed; 54.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Not finding the right product?  

Try our Product Selector Tool.

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service