MABT219
Anti-Actin Antibody, clone JLA20
clone JLA20, from mouse
Synonym(s):
Actin, alpha skeletal muscle, Alpha-actin-1
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-Actin Antibody, clone JLA20, clone JLA20, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
JLA20, monoclonal
species reactivity
human
species reactivity (predicted by homology)
chicken (immunogen homology)
technique(s)
immunofluorescence: suitable
western blot: suitable
isotype
IgMκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ACTA1(58)
Analysis Note
Control
SK-BR3 cell lysate
SK-BR3 cell lysate
Evaluated by Western Blot in SK-BR3 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Actin on 10 µg of SK-BR3 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Actin on 10 µg of SK-BR3 cell lysate.
Application
Detect Actin using this Anti-Actin Antibody, clone JLA20 validated for use in Western Blotting, Immunofluorescence.
Immunofluorescence Analysis: A representative lot was used by an independent laboratory in NRK cells. (Cao, L.G., et al. (1993). The Journal of Cell Biology. 123(1):173-181.)
Western Blot Analysis: A representative lot was used by an independent laboratory in Me4405 melanoma cell lysate. (Thomas, W.D., et al. (2000). Journal of Immunology. 165:5612–5620.)
Western Blot Analysis: A representative lot was used by an independent laboratory in Me4405 melanoma cell lysate. (Thomas, W.D., et al. (2000). Journal of Immunology. 165:5612–5620.)
General description
Actin is an abundantly expressed in eukaryotic cells and it is the core component of the cytoskeleton. It exists in three isoforms: alpha actin, which is found in muscle tissue and forms fibrous actin that facilitates muscle contractility; and beta and gamma actin which are found in non-muscle cells and facilitate cell motility. Actin exists initially as globular actin (G-actin) which is converted to filamentous actin (F-actin) upon assembly. The formation of F-actin is a complex multistep process that is tightly regulated by signaling from the actin-binding proteins (ABPs) and Rho family GTPases. Filamentous actin is crosslinked with other actin polymers to form a complex cytoskeletal network which is required for exo- and phagocytosis, vesicular trafficking, cell migration, and gene regulation, among other cellular processes.
~42 kDa observed
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1040
Physical form
Format: Purified
Not finding the right product?
Try our Product Selector Tool.
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Chunhua Zhang et al.
Frontiers in plant science, 4, 238-238 (2013-07-23)
In plant cells the actin cytoskeleton adopts many configurations, but is best understood as an unstable, interconnected track that rearranges to define the patterns of long distance transport of organelles during growth. Actin filaments do not form spontaneously; instead filament
Tanja Spoettl et al.
World journal of gastroenterology, 13(7), 1032-1041 (2007-03-22)
To examine the factor(s) involved in differentiation of intestinal macrophages (IMACs) using a recently established in vitro model. To test whether soluble or membrane bound factors induce IMAC-differentiation, freshly elutriated monocytes (MO) were incubated with conditioned media or cell membranes
David J Hughes et al.
Journal of virology, 86(2), 1034-1045 (2011-11-11)
Establishment of persistent Epstein-Barr virus (EBV) infection requires transition from a program of full viral latency gene expression (latency III) to one that is highly restricted (latency I and 0) within memory B lymphocytes. It is well established that DNA
Lívia X S Farah et al.
American journal of physiology. Renal physiology, 310(2), F123-F127 (2015-10-09)
Glucagon like peptide-1 (GLP-1) is an incretin hormone constantly secreted from the intestine at low basal levels in the fasted state; plasma concentrations rise rapidly after nutrient ingestion. Upon release, GLP-1 exerts insulinotropic effects via a G protein-coupled receptor, stimulation
Isabelle Dietrich et al.
mSphere, 2(3) (2017-05-13)
The emerging bunyavirus Rift Valley fever virus (RVFV) is transmitted to humans and livestock by a large number of mosquito species. RNA interference (RNAi) has been characterized as an important innate immune defense mechanism used by mosquitoes to limit replication
Related Content
New Products: Antibodies, Assays, Small Molecules, Inhibitors, and Proteins
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service