MABT188
Anti-Dynamin-1/2 Antibody, clone Hudy-1
clone Hudy-1, from mouse
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Dynamin-1, Dynamin, Dynamin-1/2
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
Hudy-1, monoclonal
species reactivity
human, rat
technique(s)
immunofluorescence: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... DNM1(1759)
General description
Dynamin-1 & Dynamin-2 belong to the dynamin superfamily of large GTPases. Dynamins are multidomain proteins consisting of a GTP-binding domain, a middle domain, and a GTP-effector domain. Dynamins also contain a pleckstrin homology (PH) domain which may facilitate membrane binding. Dynamin-1 is highly abundant in the brain particularly at synaptic contacts, whereas Dynamin-2 is ubiquitously expressed in non-neuronal cells. Dynamins are involved in receptor-mediated endocytosis of clathrin-coated vesicles. Dynamin-1 interacts with an array of cellular targets via its SH3 binding domain and is implicated in late stages of vesicle formation by forming oligomers around the “neck” of budding vesicles and inducing membrane fission; it may also play a role in cytoskeleton rearrangement and cytoskinesis. Dynamin-2 is involved in the formation of clathrin-coated vesicles but may also be involved in the recycling of receptors between endosomes and the trans-Golgi network.
Specificity
This antibody detects both Dynamin-1 and Dynamin-2. (Nicoziani, P., et al. (1999). Molecular Biology of the Cell. 11:481–495.)
Immunogen
Recombinant full length human Dynamin-1 expressed in transfected Sf9 cells.
Application
Anti-Dynamin-1/2 Antibody, clone Hudy-1 detects level of Dynamin-1/2 & has been published & validated for use in Western Blotting, Immunofluorescence.
Immunofluorescence Analysis: A representative lot was used by an independent laboratory in HeLa cells (Nicoziani, P., et al. (1999). Molecular Biology of the Cell. 11:481–495.)
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Quality
Evaluated by Western Blot in rat brain tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Dynamin-1/2 on 10 µg of rat brain tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Dynamin-1/2 on 10 µg of rat brain tissue lysate.
Target description
~97 kDa observed.
Dynamin 1 has a calculated MW of 97 kDa Dynamin 2 has a calcluated MW of 98 kDa
Dynamin 1 has a calculated MW of 97 kDa Dynamin 2 has a calcluated MW of 98 kDa
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4) and 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat brain tissue lysate
Rat brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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