MABT167
Anti-Transgelin/SM22a Antibody, clone 20F2.2
clone 20F2.2, from mouse
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Transgelin, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha, SM22a
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
20F2.2, monoclonal
species reactivity
human, mouse, rat
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TAGLN(6876)
General description
Transgelin, also known as 22 kDa actin-binding protein, Protein WS3-10, and Smooth muscle protein 22-alpha (SM22-alpha), is an actin cross-linking protein that is involved in cellular calcium interactions and contractile properties of the cytoskeleton contributing to replication and senescence in smooth muscle tissue. Transgelin is overexpressed in senescent human fibroblasts.
Immunogen
GST-tagged recombinant protein corresponding to human Transgelin/SM22a.
Application
Anti-Transgelin/SM22a Antibody, clone 20F2.2 is a highly specific mouse monoclonal antibody, that targets Transgelin & has been tested in western blotting & ICC.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Western Blotting Analysis: 1 µg/ml from a representative lot detected Transgelin/SM22a in 10 µg of NIH/3T3 and PC12 cell lysates.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Transgelin/SM22a in NIH/3T3 cells.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Transgelin/SM22a in NIH/3T3 cells.
Quality
Evaluated by Western Blotting in U20S cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Transgelin/SM22a in 10 µg of U20S cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Transgelin/SM22a in 10 µg of U20S cell lysate.
Target description
~22 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
U20S cell lysate
U20S cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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