Anti-TSH Receptor Antibody, clone CS-17

Thyrotropin receptor (UniProt: P16473; also known as Thyroid-stimulating hormone receptor, TSH-R) is encoded by the TSHR (also known as LGR3) gene (Gene ID: 7253) in human. TSH-R is a multi-pass membrane protein of the G-protein coupled receptor (GPCR) family that is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to generate the mature form that contains four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. The extracellular domain is linked to a distal signal-specific domain the hinge region that is attached to a transmembrane domain consisting of extracellular and intracellular loops. It serves as a receptor the thyroid-stimulating hormone and TSH binding to this receptor induces thyrocyte growth and proliferation primarily by activating the adenylate cyclase signaling pathway. Clone CS-17 acts allosterically and serves as an inverse agonist for TSH-R constitutive activity. When administered in vivo it is shown to suppress thyroid function and reduces serum T4 levels in BALB/c mice. (Ref.: Chen, RR., et al. (2018). Thyroid. 28(77); 933-940; Chen, CR., et al. (2007). Endocrinology. 148(5); 2375-2382).

Clone CS-17 is a mouse monoclonal antibody that detects human Thyrotropin receptor. It targets an epitope within the first extracellular domain from the N-terminal region.

Adenovirus expressing TSH receptor fragment (aa 22-289). adenovirus.

Quality Control Testing

Evaluated by Flow Cytometry in TSH-R transfected HEK293 cells.

Flow Cytometry Analysis: 1 μg of this antibody detected TSH Receptor in one million TSH-R transfected HEK293 cells.

Tested Applications

Flow Cytometry: A representative lot detected TSH Receptor in Flow Cytometry applications (Chen, C.R., et al. (2007). Endocrinology. 148(5):2375-82; Chen, C.R., et al. (2009). Endocrinology. 150(7):3401-8; Chen, C.R., et al. (2018). Thyroid. 28(7):933-940).

Inhibition: A representative lot inhibited the constitutive activity of the TSH Receptor. (Furmaniak, J. et al. (2012). Autoimmun Highlights. 4(1):11-26).

Radioimmunoassay: A representative lot detected TSH Receptor in Radioimmunoassay applications (Chen, C.R., et al. (2007). Endocrinology. 148(5):2375-82).

Antagonist Function: A representative lot bloced TSH binding to the TSH Receptor. Endocrinology. 150(7):3401-8; Chen, C.R., et al. (2018). Thyroid. 28(7):933-940).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-TSH Receptor, clone CS-17, Cat. No. MABS2240, is a mouse monoclonal antibody that detects Thyroid-stimulating hormone receptor (TSH-R) and is tested for use in Flow Cytometry, Antagonist Function, Inhibition Assay, and Radioimmunoassay.

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.