Anti-Osteocalcin (Glu17) Antibody, clone 5C5

Osteocalcin (UniProt: P02818; also known as Bone Gla protein, BGP, Gamma-carboxyglutamic acid-containing protein) is encoded by the BGLAP gene (Gene ID: 632) in human. Osteocalcin is a member of the osteocalcin/matrix Gla protein family and can constitute 1 to 2% of the total bone protein. It is synthesized by osteoblasts and is a major component of the noncollagenous bone matrix. Osteocalcin is synthesized with a signal peptide (aa 1-23), a propetide (aa 24-51) that are cleaved to generate active secreted form of osteocalcin (aa 52-100). Osteocalcin displays a high affinity for calcium and binds strongly to apatite. Sixty to ninety percent of de novo synthesized osteocalcin is incorporated into the bone matrix where it binds to hydroxyapatite during matrix mineralization. The remainder of the osteocalcin is released into the circulation where it can be measured as a sensitive marker of bone formation. Osteocalcin found in serum is almost exclusively derived from the bone formation with little or no contribution from the resorption process. A major feature of osteocalcin is the presence of 3 vitamin K-dependent gamma-carboxyglutamic acid (Gla) residues, which are found in the first helical region and interact with the inter-calcium spacing in the HAP lattice. In humans, the percentage of the circulating osteocalcin that is not gamma-carboxylated is used as a biomarker of vitamin K status. Osteocalcin expression in osteoblasts is stimulated by insulin and adiponectin and osteocalcin enhances insulin sensitivity and differentiation in muscle. Serum osteocalcin is elevated in diseases characterized by increased bone turnover such as osteoporosis, hyperparathyroidism and Paget′s disease, and low in conditions associated with low bone turnover such as hypoparathyroidism and growth hormone deficiency. (Ref.: Kanazawa I (2015). World J Diabetes. 6(18): 1345 1354; Gundberg, CM et al. (2012). Adv Nutr 3 (2): 149-157).

Clone 5C5 detects human osteocalcin only when not carboxylated on the first glutamic acid residue (GLU17). It does not react with fully carboxylated osteocalcin.

A full length recombinant fragment corresponding to 49 amino acids of the mature decarboxylated osteocalcin (with signal peptide and pro regions cleaved off).

Epitope: domain of:

Anti-Osteocalcin (Glu17), clone 5C5, Cat. No. MABS1978, is a mouse monoclonal antibody that detects decarboxylated osteocalcin and has been tested for use in Dot Blot and Enzyme Immunoassay (ELISA).

Dot Blot Analysis: A representative lot detected Osteocalcin (Glu17) in 3xGlu, Glu17, and 3XGla peptides for human OCN (Courtesy of Mathieu Ferron, Ph.D., Montreal Clinical Research Institute, Montreal, Canada).

Enzyme Immunoassay Analysis: A representative lot detected Osteocalcin (Glu17) in human serum.

Research Category
Signaling

Dot Blot Analysis: A 1:250 dilution from a representative lot detected decaboxylated Osteocalcin (Glu17) at a wide range of concentrations (0.8 to 1600 ng).

10.96 kDa calculated.

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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