MABS1914
Anti-Mitochondrial Pyruvate Carrier 2 (BRP44)
clone n/a, from mouse
Sign Into View Organizational & Contract Pricing
All Photos(2)
Mitochondrial pyruvate carrier 2, Brain protein 44, BRP44
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
n/a, monoclonal
species reactivity
mouse, human
species reactivity (predicted by homology)
rat (based on 100% sequence homology)
packaging
antibody small pack of 25 μg
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... MPC2(25874)
General description
Mitochondrial pyruvate carrier 2 (UniProt: O95563; also known as Brain protein 44, MPC2) is encoded by the MPC2 (also known as BRP44) in human. MPC2 is a multi-pass inner mitochondrial membrane protein that mediates the imports pyruvate into mitochondria. Pyruvate is an essential component in intermediary metabolism that occupies a key crossroads position between cytosolic glycolysis and mitochondrial metabolism. MPC2 contains 3 helical domains (aa 41-61; 73-90; and 96-115). In mammalian cells mitochondrial pyruvate carrier is composed on two paralogous subunits known as MPC1 and MPC2 that heteromerize to form a functional complex. It has been reported that MPC2 can also homomerize, however only MPC1/MPC2 heteromeric forms are functional. Complete absence of MPC2 is shown to cause embryonic lethality in murine models, however, murine cell lines that express an N-terminal truncated MPC2 protein (Mpc2 16) are reported to be viable, but exhibit significantly reduced capacity for mitochondrial pyruvate oxidation. (Ref.: Compan, V., et al. (2015). Mol. Cell 59(3); 491-501; Vigueira, PA., et al. (2014). Cell Rep. 7(6); 2042-2053).
Specificity
This mouse monoclonal antibody detects Mitochondrial Pyruvate carrrier Protein 2 in human and mouse cells. It targets an epitope within 10 amino acids from the internal region.
Immunogen
A linear peptide corresponding to 10 amino acids from the internal region of human mitochondrial pyruvate carrier 2 protein.
Application
Anti-Mitochondrial Pyruvate Carrier 2 (BRP44), Cat. No. MABS1914, is a mouse monoclonal antibody that detects human and mouse Mitochondrial pyruvate carrier 2 and has been tested for use in Immunoprecipitation and Western Blotting.
Research Category
Signaling
Signaling
Western Blotting Analysis: 1 µg/mL from a representative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse heart and mouse liver tissue lysates.
Immunoprecipitation Analysis: A 1:1,000 dilution from a representative lot immunoprecipitated Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse liver mitoplasts (Courtesy of Prof. Jean-Claude Martinou, Ph.D., University of Geneva, Switzerland)..
Western Blotting Analysis: 4 µg/mL from a represenetative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse pancreas tissue lysate.
Western Blotting Analysis: A representative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in Western Blotting applications (Compan, V., et. al. (2015). Mol Cell. 59(3):491-501; Vanderperre, B., et. al. (2016). PLoS Genet. 12(5):e1006056).
Immunoprecipitation Analysis: A 1:1,000 dilution from a representative lot immunoprecipitated Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse liver mitoplasts (Courtesy of Prof. Jean-Claude Martinou, Ph.D., University of Geneva, Switzerland)..
Western Blotting Analysis: 4 µg/mL from a represenetative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse pancreas tissue lysate.
Western Blotting Analysis: A representative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in Western Blotting applications (Compan, V., et. al. (2015). Mol Cell. 59(3):491-501; Vanderperre, B., et. al. (2016). PLoS Genet. 12(5):e1006056).
Quality
Evaluated by Western Blotting in mouse liver mitochondrial lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse liver mitochondrial lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse liver mitochondrial lysate.
Target description
~15 kDa observed; 14.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Frontiers in cardiovascular medicine, 5, 195-195 (2019-02-12)
Introduction: Group 2 pulmonary hypertension (PH), defined as a mean pulmonary arterial pressure ≥25 mmHg with elevated pulmonary capillary wedge pressure >15 mmHg, has no approved therapy and patients often die from right ventricular failure (RVF). Alterations in mitochondrial metabolism
Cells, 11(7) (2022-04-13)
The oncogenic expression or mutation of tumor suppressors drives metabolic alteration, causing cancer cells to utilize diverse nutrients. Lactate is a known substrate for cancer cells, yet the regulatory mechanisms of lactate catabolism are limited. Here, we show that a
Paradoxical neuronal hyperexcitability in a mouse model of mitochondrial pyruvate import deficiency.
eLife, 11 (2022-02-22)
Neuronal excitation imposes a high demand of ATP in neurons. Most of the ATP derives primarily from pyruvate-mediated oxidative phosphorylation, a process that relies on import of pyruvate into mitochondria occuring exclusively via the mitochondrial pyruvate carrier (MPC). To investigate
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service