Anti-R-Spondin 2 Antibody, clone 14C7.1

R-spondin-2 (UniProt: Q6UXX9; also known as Roof plate-specific spondin-2, hRspo2) is encoded by the RSPO2 (also known as UNQ9384/PRO34209) gene (Gene ID: 340419) in human. R-spondin-2 is a secreted protein that acts as an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR receptors. Upon binding to LGR receptors (LGR4, LGR5 or LGR6) these receptors associate with phosphorylated LRP6 and frizzled receptors, which are activated by extracellular Wnt receptors. These events trigger the canonical Wnt signaling pathway to increase expression of target genes. R-spondin-2 also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling pathway by acting as an inhibitor of ZNRF3, an E3 ubiquitin-protein ligase that serves as an important regulator of the Wnt signaling pathway. In humans, R-Spondin 2 is expressed in organs of endodermal origin in adults, including intestine and lungs and is reported to downregulated in tumors involving these organs. In the embryonic mice it is abundantly expressed in the hippocampus and in developing bones.

Clone 14C7.1 specifically detects human R-spondin-2. It targets an epitope within 74 amino acids from the C-terminal end.

GST/His-tagged recombinant fragment corresponding to 74 amino acids from the C-terminal end of human R-Spondin 2.

Anti-R-Spondin 2, clone 14C7.1, Cat. No. MABS1709, is a mouse monoclonal antibody that specifically targets R-Spondin 2 (RSPO2) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected RSPO2 in human pancreas, human kidney, and human bone marrow tissue.

Research Category
Signaling

Evaluated by Western Blotting in human recombinant R-spondin 2 protein.

Western Blotting Analysis: 0.02 µg/mL of this antibody detected 0.05 µg of human recombinant R-spondin 2 protein.

~35 kDa observed; 28.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.