Anti-Thrombomodulin Antibody, clone 411-201B

Thrombomodulin (UniProt P15306; also known as CD141, Fetomodulin, SnoRNA MBII-339, TM) is encoded by the Thbd gene (Gene ID 21824) in murine species. Thrombomodulin (TM) is an endothelial cell surface protein that functions to suppress the propagation of thrombin (activated blood-coagulation factor II) generation by acting as a thrombin receptor. Formation of the TM-thrombin complex abolishes thrombin′s procoagulant activity by altering its substrate specificity, leading to proteolytic cleavage of protein C into the natural anticoagulant termed "activated protein C". Together with protein S, activated protein C proteolytically cleaves activated coagulation factors Va and VIIIa, thereby preventing the generation of activated coagulation factors Xa and thrombin. Murine thrombomodulin is initially produced with an N-terminal propeptide (a.a. 1-16) sequence, the removal of which yields the mature protein with a large extracellular region (a.a. 17-517), a transmembrane segment (a.a. 518-541), and a short cytoplamic tail (a.a. 542-577).

Epitope: Extracellular domain.

Normal mouse lung homogenate.

Anti-Thrombomodulin Antibody, clone 411-201B is an antibody against Thrombomodulin for use in Immunohistochemistry (Paraffin), Electron Microscopy, Immunoprecipitation, Western Blotting, ELISA.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Thrombomodulin in human kidney tissue.
Electron Microscopy: A representative lot detected thrombomodulin immunoreactivity associated with capillary endothelial cells by EM using frozen mouse lung sections (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701).
Immunoprecipitation Analysis: A representative lot immunoprecipitated the 112 kDa thrombomodulin from normal mouse lung fibroblast (NMLF) lysates, but not from thrombomodulin-depleted NMLF lysates by performing IP with clone 273-34A (Cat. No. MABS1245) prior to IP with clone 411-201B (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701).
Western Blotting Analysis: A representative lot detected thrombomodulin (P112) in the normal mouse lung fibroblast (NMLF) immunoprecipitates obtained with either clone 411-201B or clone 273-34A (Cat. No. MABS1245) (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701).
Immunohistochemistry Analysis: Representative lots and clone 273-34A (Cat. No. MABS1245) showed similar staining pattern using both frozen and paraffin-embedded mouse lung sections (Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450; Rorvik, M.C., et al. (1988). J. Histochem. Cytochem. 36(7):741-749; Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701).
ELISA Analysis: Representative lots were used as the detection antibody for the detection of thrombomodulin in mouse tissue samples by sandwich ELISA using clone 273-34A (Cat. No. MABS1245) as the capture antibody and an HRP-conjugated secondary antibody, or by sandwich radio-immunoassay employing 125I-labeled clone 411-201B without an enzyme-conjugated secondary antibody (Isermann, B., et al. (2001). Development. 128(6):827-838; Conway, E.M., et al. (1999). Blood. 93(10):3442-3450; Weiler-Guettler, H., et al. (1996). Circ. Res. 78(2):180-187; Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450).

Research Category
Signaling

Research Sub Category
Immunoglobulins & Immunology

Evaluated by Immunohistochemistry in mouse lung tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Thrombomodulin in mouse lung tissue.

61.87 kDa calculated.

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.