MABS1250
Anti-Mineralocorticoid Receptor Antibody, clone 2D6
clone 2D6, from mouse
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MR, Nuclear receptor subfamily 3 group C member 2, Mineralocorticoid Receptor
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2D6, monoclonal
species reactivity
rat
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
rat ... Nr3C2(25672)
General description
Mineralocorticoid receptor (UniProt P22199; also known as MR, Nuclear receptor subfamily 3 group C member 2) is encoded by the Nr3c2 (also known as MCR, Mlr) gene (Gene ID 25672) in rats. Mineralocorticoid receptor (MR) is expressed in multiple tissues, including renal epithelia, smooth muscle, endothelium, cardiomyocytes, and hippocampal neurons, where it mediates diverse functions. MR is activated by aldosterone and also by cortisol in cells that do not express 11β-hydroxysteroid dehydrogenase type 2 (11βHSD2). MR is normally activated by aldosterone, which is produced by adrenal glomerulosa in response to intravascular volume depletion and hyperkalemia. Consistently, gain of function mutations in MR lead to severe hypertension, often with hypokalemia, while loss of function mutations result in neonatal hypotension. Ser843 phosphorylation in the MR ligand-binding domain is reported to prevent MR ligand binding and activation. MR pS843 is found exclusively in intercalated cells of the distal nephrons in the kidney. Intravascular volume depletion, angiotensin II and WNK4 signaling reduce MR pS843 levels, whereas hyperkalemia increases MR pS843.
Specificity
Epitope is present in all 3 alternative spliced isoforms
Immunogen
Conjugated linear peptide corresponding to the N-terminus of rat Mineralocorticoid Receptor.
Epitope: Near N-terminus
Application
Detect Mineralocorticoid Receptor using this Anti-Mineralocorticoid Receptor Antibody, clone 2D6 validated for use in Western Blotting, Immunohistochemistry and Immunocytochemistry.
Immunohistochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in hippocampus, dentate gyrate, choroid plexus, heart, and rat kidney (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
Immunocytochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in mouse kidney (Shibata, S., et al. (2013). Cell Metabolism. 18:660-671).
Western Blotting Analysis: A representative lot detected endogenous Mineralocorticoid Receptor (MR) in rat hippocampal cytosolic preparation and exogenously expressed EGFP-rat MR fusion protein in CHO cells (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
Western Blotting Analysis: A representative lot detected endogenous Mineralocorticoid Receptor (MR) in mouse kidney cytosolic fraction and exogenously expressed human MR in COS-7 cells (Shibata, S., et al. (2013) Cell Metab. 18(5):660-671)
Immunocytochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in mouse kidney (Shibata, S., et al. (2013). Cell Metabolism. 18:660-671).
Western Blotting Analysis: A representative lot detected endogenous Mineralocorticoid Receptor (MR) in rat hippocampal cytosolic preparation and exogenously expressed EGFP-rat MR fusion protein in CHO cells (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
Western Blotting Analysis: A representative lot detected endogenous Mineralocorticoid Receptor (MR) in mouse kidney cytosolic fraction and exogenously expressed human MR in COS-7 cells (Shibata, S., et al. (2013) Cell Metab. 18(5):660-671)
Research Category
Signaling
Signaling
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Quality
Evaluated by Western Blotting in rat brain cytosol tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Mineralocorticoid Receptor in 10 µg of rat brain cytosol tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Mineralocorticoid Receptor in 10 µg of rat brain cytosol tissue lysate.
Target description
~107 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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