MABS1225
Anti-TXNIP Antibody, clone JY2
clone JY2, from mouse
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Synonym(s):
Thioredoxin-interacting protein, Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1
UNSPSC Code:
12352203
eCl@ss:
32160702
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
JY2, monoclonal
species reactivity
human, mouse
technique(s)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... TXNIP(10628)
General description
Thioredoxin-interacting protein (UniProt Q9H3M7; also known as Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1) is encoded by the TXNIP (also known as VDUP1) gene (Gene ID 10628) in human. Thioredoxin interacting protein (TXNIP) is an alpha-arrestin protein that acts as an oxidative stress mediator that binds thioredoxin active cysteine residue and suppresses its antioxidant activity. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. TXNIP over-expression results in increased cellular ROS level and oxidative cell death, while TXNIP suppression enhances cellular resistance to oxidative injury. TXNIP is also shown to inhibit cellular glucose uptake by downregulating plasma membrane localization of glucose transporter 1 (GLUT1). TXNIP is required for the maturation of natural killer cells. Its expression is markedly down-regulated in tumor cells and its levels are correlated with clinical stage of cancer. Mice with mutations or knockout of TXNIP gene are much more susceptible to carcinogenesis than wild-type mice, indicating its role in cancer suppression. Ref.: Masutani, H., et al. (2012). J. Clin. Biochem. Nutr. 50, 23-34.
Specificity
Clone JY2 detected TXNIP in adult mouse cardiomyocytes. No TXNIP target band was detected in cardiomyocytes from Txnip-null mice (Yoshioka, J., et al. (2007). Circ. Res. 101(12):1328-1338).
Immunogen
Recombinant full-length human TXNIP protein.
Application
Detect TXNIP/VDUP1 using this mouse monoclonal Anti-TXNIP Antibody, clone JY2, Cat. No. MABS1225, validated for use in Western Blotting.
Research Category
Signaling
Signaling
Western Blotting Analysis: A representative lot detected full-length human TXNIP, but not human TXNIP constructs lacking C-terminal end a.a. 302-391 sequence, exogenously expressed in HEK293 cells (Yoshioka, J., et al. (2007). Circ. Res. 101(12):1328-1338).
Western Blotting Analysis: A representative lot detected TXNIP in adult mouse cardiomyocytes. No TXNIP target band was detected in cardiomyocytes from Txnip-null mice (Yoshioka, J., et al. (2007). Circ. Res. 101(12):1328-1338).
Western Blotting Analysis: A representative lot detected TXNIP in adult mouse cardiomyocytes. No TXNIP target band was detected in cardiomyocytes from Txnip-null mice (Yoshioka, J., et al. (2007). Circ. Res. 101(12):1328-1338).
Quality
Evaluated by Western Blotting in HeLa cells.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected TXNIP in 10 µg of HeLa cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected TXNIP in 10 µg of HeLa cell lysate.
Target description
~53 kDa observed. 43.66/37.32 kDa (human isoform 1/2) and 44.36/44.29 kDa (mouse isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified.
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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