MABS1214
Anti-PEG (methoxy group) Antibody, clone RM105
clone RM105, from rabbit, purified by affinity chromatography
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PEG, pegylation, methoxy group, Polyethylene glycol, polyethylene oxide, PEO, polyoxyethylene, POE
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biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RM105, monoclonal
purified by
affinity chromatography
species reactivity (predicted by homology)
all
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
unmodified
General description
Polyethylene glycol (PEG) is a polyether compound with many applications from industrial manufacturing to medicine. PEG is also known as polyethylene oxide (PEO) or polyoxyethylene (POE), depending on its molecular weight. PEG is a family of long chain polymers attached to a glycerine backbone. Some of the properties of PEG are nonionic, nontoxic, biocompatible, strongly hydrophilic polymer, which has a large exclusion volume in aqueous solution. PEGylation is the act of covalently coupling a PEG structure to another larger molecule, for example, a therapeutic protein, which is then referred to as a PEGylated protein. The covalent attachment of PEG (PEGylation) is commonly used to modify a variety of proteins and drugs. Some of the therapeutical benefits of PEGylated molecules include increased bioavailability, increased blood circulation of the drug, optimized pharmacokinetics, decreased immunogenicity, and decreased frequency of administration. Additional benefits of PEGylated molecules include decelerated renal excretion, improved stability towards proteolysis, increased solubility of the biopharmaceutical in aqueous solutions, increased hydrodynamic radius, reduced immunogenicity and proteolytic cleavage. This antibody reacts to the terminal methoxy group of Polyethylene glycol.
Immunogen
recombinant protein corresponding to All PEG (methoxy group).
Application
Anti-PEG (methoxy group), clone RM105 is an antibody against PEG (methoxy group) for use in WB, IH, ELISA.
Immunohistochemistry Analysis: 0.5 μg/mL of this antibody detected PEG (methoxy)-BSA in injected mouse liver and kidney
ELISA Analysis: Multiple concentrations of this antibody was used to detect three different BSA-PEG conjugates (5K, 40K and 40K branched)
ELISA Analysis: Multiple concentrations of this antibody was used to detect three different BSA-PEG conjugates (5K, 40K and 40K branched)
Research Category
Signaling
Signaling
Research Sub Category
Epitope Tags
Epitope Tags
Quality
Evaluated by Western Blotting on recombinant methoxyPEG conjugated BSA
Western Blotting Analysis: A 1:1;000 dilution of this antibody detected PEG (methoxy group) on conjugated recombinant BSA
Western Blotting Analysis: A 1:1;000 dilution of this antibody detected PEG (methoxy group) on conjugated recombinant BSA
Target description
BSA is 66 kD, methoxyPEG is 5 kD. Additional bands above 66 kD is due to different levels of conjugation and dimerization.
Physical form
Protein A purified
Purified rabbit monoclonal IgG in PBS buffer containing 1% BSA and 0.09% sodium azide
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Certificates of Analysis (COA)
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