MABS1132
Anti-CDKL5 Antibody, clone 8F3.1
clone 8F3.1, from mouse
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Synonym(s):
Cyclin-dependent kinase-like 5, Serine/threonine-protein kinase 9
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
8F3.1, monoclonal
species reactivity
mouse, rat
species reactivity (predicted by homology)
human (based on 100% sequence homology)
technique(s)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Cdkl5(382253)
General description
Cyclin-dependent kinase-like 5 (EC 2.7.11.22; UniProt O76039; also known as Serine/threonine-protein kinase 9) is encoded by the CDKL5 (also known as EIEE2, STK9) gene (Gene ID 6792) in human. CDKL5/STK9 is a serine/threonine protein kinase widely distributed in all tissues, with highest levels in thymus, testes, and brain, where it is found localized in the nucleus and dendrite of neurons. CDKL5/STK9 expression is found peaked in the early postnatal period and Cdkl5-knockdown in mice leads to autistic-like behavior, dendritic hypotrophy and impairment of neural circuit communication. Likewise, human CDKL5 gene mutations are linked to X-linked neurological developmental disorders involving severe mental retardation and seizures appearing in the first postnatal months. Point mutations in CDKL5 have also been reported in patients with atypical variants of Rett syndrome (RTT), infantile spasms and severe neurodevelopmental retardation.
Specificity
Expected to react with both spliced isoforms of human CDKL5 reported by UniProt (O76039).
Immunogen
Epitope: Internal.
GST-tagged recombinant human CDKL5 internal fragment.
Application
Anti-CDKL5 Antibody, clone 8F3.1 is an antibody against CDKL5 for use in Western Blotting.
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected CDKL5 in 10 µg of rat hippocampus tissue lysate.
Quality
Evaluated by Western Blotting in mouse cerebellum tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected CDKL5 in 10 µg of mouse cerebellum tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected CDKL5 in 10 µg of mouse cerebellum tissue lysate.
Target description
~115 kDa observed. 115.5 kDa (isoform 1) and 107.5 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Ji Young Kim et al.
Nature communications, 11(1), 1924-1924 (2020-04-23)
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