MABS107-I
Anti-Ubiquitin K11 linkage Antibody, clone 2A3/2E6
clone 2A3/2E6, from rabbit
Synonym(s):
Ubiquitin K11 linkage, Lysine 11
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2A3/2E6, monoclonal
species reactivity
human
species reactivity (predicted by homology)
all (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... UBAP2(55833)
General description
Recently, the lysine11 (K11) linkage of ubiquitin has been shown to be specifically targeted by the Anaphase-Promoting Complex (APC) E3 ubiquitin ligase for catalyzed ubiquitination for mitosis. Antibodies specific to the K11-linkage have shown that the formation of the K11 chains are greatly increased in mitotic human cells in the presence of APC substrate degradation. K11-linked ubiquitin chains seem to have essential regulatory control over mitotic protein degradation.
Immunogen
Recombinant protein corresponding to all in Ubiquitin K11 linkage.
Application
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Ubiquitin K11 linkage in A431 and HeLa cells.
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
This Anti-Ubiquitin K11 linkage Antibody, clone 2A3/2E6 is validated for use in western blotting & ICC for the detection of Ubiquitin K11 linkage.
Quality
Evaluated by Western Blotting in K11 recombinant protein.
Western Blotting Analysis: A 1:250 to 1:1,000 dilution of this antibody detected Ubiquitin K11 linkage in 10 µg of K11 recombinant protein.
Western Blotting Analysis: A 1:250 to 1:1,000 dilution of this antibody detected Ubiquitin K11 linkage in 10 µg of K11 recombinant protein.
Target description
Varies
Physical form
Format: Purified
Protein A purified
Purified rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
新产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Mingwei Min et al.
Molecular biology of the cell, 26(24), 4325-4332 (2015-10-09)
The ubiquitin proteasome system (UPS) directs programmed destruction of key cellular regulators via posttranslational modification of its targets with polyubiquitin chains. These commonly contain Lys-48 (K48)-directed ubiquitin linkages, but chains containing atypical Lys-11 (K11) linkages also target substrates to the
Rahul S Samant et al.
Nature, 563(7731), 407-411 (2018-11-16)
Protein misfolding is linked to a wide array of human disorders, including Alzheimer's disease, Parkinson's disease and type II diabetes1,2. Protective cellular protein quality control (PQC) mechanisms have evolved to selectively recognize misfolded proteins and limit their toxic effects3-9, thus
Michael E French et al.
The Journal of biological chemistry, 292(25), 10398-10413 (2017-05-04)
Homologous to E6AP C-terminal (HECT) ubiquitin (Ub) ligases (E3s) are a large class of enzymes that bind to their substrates and catalyze ubiquitination through the formation of a Ub thioester intermediate. The mechanisms by which these E3s assemble polyubiquitin chains
Swarna L Vijayaraj et al.
Nature communications, 12(1), 2713-2713 (2021-05-13)
Interleukin-1β (IL-1β) is activated by inflammasome-associated caspase-1 in rare autoinflammatory conditions and in a variety of other inflammatory diseases. Therefore, IL-1β activity must be fine-tuned to enable anti-microbial responses whilst limiting collateral damage. Here, we show that precursor IL-1β is
Animesh Dhara et al.
mSphere, 1(3) (2016-06-25)
The contribution of ubiquitin-mediated mechanisms in the regulation of the Toxoplasma gondii cell cycle has remained largely unexplored. Here, we describe the functional characterization of a T. gondii deubiquitinase (TGGT1_258780) of the ovarian-tumor domain-containing (OTU) family, which, based on its structural
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