Anti-NB-2/Cntn5 Antibody, clone 1A6

Contactin-5 (UniProt P68500; also known as Neural recognition molecule NB-2) is encoded by the Cntn5 gene (Gene ID 244682) in murine species. Contactin-5 (NB-2) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein belonging to a subgroup of the immunoglobulin superfamily of proteins known as contactins. Five of the six contactins, Contactin-1, contactin-2/TAG-1, contactin-3/BIG-1, contactin-4/BIG-2, and contactin-5/NB-2 are expressed by DRG neurons. Of these, contactin-1, TAG-1, and BIG-2, are also expressed by motor neurons. NB-2 plays an important role in synapse formation in the developing auditory system in rodents. Expression of NB-2 is seen in the central auditory pathways just before the onset of hearing in rodents and is localized at glutamatergic synapses in the postnatal auditory brainstem. NB-2-knockout mice exhibit abnormal responses to auditory stimuli in both neuronal excitability and behavior, and have delayed auditory brainstem response waves. In addition, NB-2 deficiency in mice causes a significant reduction in the number of synapses and induces apoptosis in a subset of neurons in the auditory brainstem. NB-2 is produced with an N-terminal signal peptide (a.a. 1-23) and a C-terminal propeptide (a.a. 1072-1098) sequence, the removal of which yields the mature GPI-anchored glycoprotein (a.a. 24-1071) with six C2-type Ig-like domains (a.a. 98-659) and four type-III fibronectin domains (a.a. 672-1066).

Clone 1A6 detected NB-2 in the synaptosomal, but not mitochondrial, fraction from mouse cerebral cortex homogenate (Shimoda, Y., et al. (2012). Neurosci. Lett. 510(2):148-153).

Human IgG Fc-tagged recombinant mouse NB-2/Cntn5.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

This Anti-NB-2 Antibody, clone 1A6 is validated for use in Western Blotting, Immunocytochemistry, Immunofluorescence for the detection of Cntn5.

Western Blotting Analysis: 2.0 ug/mL from a representative lot detected NB-2 in postnatal P14 and adult mouse brain tissue lysates (Courtesy of Dr. Yasushi Shimoda, PhD, Nagaoka University of Technology, Nagaoka, Japan).
Immunocytochemistry Analysis: A representative lot detected punctate NB-2 immunoreactivity on the dendrites of cultured embryonic E17 mouse hippocampal neurons. A partial overlap of NB-2 immunoreactivity with those of APLP1 and synapsin was seen (Shimoda, Y., et al. (2012). Neurosci. Lett. 510(2):148-153).
Immunofluorescence Analysis: A representative lot detected NB-2 immunoreactivity among parvalbumin-/PV-positive proprioceptive sensory neurons by fluorescent immunohistochemistry staining of postnatal day 6 (p6) mouse dorsal root ganglion (DRG) cryosectoins (Ashrafi, S., et al. (2014). Neuron. 81(1):120-129).
Western Blotting Analysis: A representative lot detected NB-2 in presynaptic preparations from the spinal cords of postnatal p6 to p7 mice (Ashrafi, S., et al. (2014). Neuron. 81(1):120-129).
Western Blotting Analysis: A representative lot detected NB-2 in the synaptosomal fractions from mouse cerebral cortex homogenate (Shimoda, Y., et al. (2012). Neurosci. Lett. 510(2):148-153).

Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be IgG2a.

~130 kDa observed. Target band size appears larger than the calculated molecular weights of 120.7/118.0/115.1 kDa (Prepo-/Pro-/mature NB-2) due to glycosylation.

Format: Purified

Protein G purified.

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.