MABN866
Anti-Myocilin (NT) Antibody, clone 7.1
clone 7.1, from mouse
Synonym(s):
Myocilin, Myocilin 55 kDa subunit, Trabecular meshwork-induced glucocorticoid response protein, Myocilin, N-terminal fragment, Myocilin 20 kDa N-terminal fragment
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
7.1, monoclonal
species reactivity
human, mouse
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MYOC(4653)
General description
Myocilin (mutated trabecular meshwork-induced glucocorticoid response protein) is encoded by the MYOC gene (also known as GLC1A, GPOA, JOAG, JOAG1, TIGR) in human (Entrez Gene ID 4653). Myocilin is produced as a 504 amino acid glycoprotein containing an N-terminal hydrophobic signal peptide sequence, a coiled-coiled leucine zipper domain, and a C-terminal domain with homology to olfactomedins. Myocilin is commonly identified as a 53/57 kDa doublet by gel electrophoresis, and a 66 kDa form of the protein is also reported. Myocilin is known to undergo intracellular endoproteolytic cleavage between Glu214 and Leu215, resulting in a 20 kDa N-terminal and a 35 kDa C-terminal fragment. Myocilin is identified as a Lingo-1 receptor ligand and MYOC gene mutations are linked to 10% of juvenile open-angle glaucoma cases and 3-4% of those with primary open-angle glaucoma (PMID 24732711 24741044, 24837143). Cat. No. MABN866, clone 7.1 is a mouse monoclonal antibody that recognizes an epitope within the N-terminal fragment (aa33-214) and is demonstrated to be suitable for Western blotting, immuncytochemistry, and immunhistochemistry applications. This clone is reactive toward both human and murine species. (PMID 18674535 & 23979599).
Immunogen
Epitope: N-terminal fragment (aa 33-214)
Recombinant protein corresponding to the N-terminal fragment (aa 33-214) of human Myocilin.
Application
Anti-Myocilin Antibody, clone 7.1 is an antibody against Myocilin for use in Western Blotting, Immunohistochemistry.
Immunohistochemistry Analysis: A representative lot detected Myocilin in human trabecular tissue (Ezzat, M.K., et al. (2008). Exp. Eye Res. 87(4):376-384).
Western Blotting Analysis: A representative lot detected Myocilin in human trabecular tissue (Ezzat, M.K., et al. (2008). Exp. Eye Res. 87(4):376-384).
Western Blotting Analysis: A representative lot detected Myocilin in aqueous humor (Zhao, Y., et al. (2013). Mol. Cell Biol. 33(21):4225-4240).
Western Blotting Analysis: A representative lot detected Myocilin in human trabecular tissue (Ezzat, M.K., et al. (2008). Exp. Eye Res. 87(4):376-384).
Western Blotting Analysis: A representative lot detected Myocilin in aqueous humor (Zhao, Y., et al. (2013). Mol. Cell Biol. 33(21):4225-4240).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Signaling
Developmental Signaling
Quality
Evaluated by Western Blotting in mouse eye tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Myocilin in 10 µg of mouse eye tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Myocilin in 10 µg of mouse eye tissue lysate.
Target description
~53, 60 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Maximilian Binter et al.
PloS one, 18(12), e0296124-e0296124 (2023-12-21)
The outflow pathway, especially trabecular meshwork (TM), plays an essential role in glaucoma, and the availability of TM cells is crucial for in vitro research. So far, the isolation of TM cells from mice has been anything but manageable due
Biting Zhou et al.
Frontiers in genetics, 13, 1019208-1019208 (2022-10-22)
MYOC is a common pathogenic gene for primary open-angle glaucoma and encodes the protein named myocilin. Multiple MYOC variations have been found, with different clinical significance. However, the pathogenesis of glaucoma induced by MYOC mutations has not been fully clarified.
Kate E Keller et al.
Experimental eye research, 171, 164-173 (2018-03-13)
Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the
Sarah M Judge et al.
Cancer research, 80(9), 1861-1874 (2020-03-07)
Skeletal muscle wasting is a devastating consequence of cancer that contributes to increased complications and poor survival, but is not well understood at the molecular level. Herein, we investigated the role of Myocilin (Myoc), a skeletal muscle hypertrophy-promoting protein that
Philip Mzyk et al.
International journal of molecular sciences, 23(4) (2022-02-27)
Although the extracellular matrix (ECM) in trabecular meshwork (TM) cells is known to be important in intraocular pressure (IOP) regulation, the molecular mechanisms involved in generating a glaucomatous environment in the TM are not completely understood. Recently we identified a
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