MABN816
Anti-Ninjurin-1/NINJ1 Antibody, clone 12H6.1
clone 12H6.1, from mouse
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Ninjurin-1, Nerve injury-induced protein 1
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
12H6.1, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NINJ1(4814)
General description
Ninjurin-1 (UniProt Q92982; also known as Nerve injury-induced protein 1) is encoded by the NINJ1 (also known as NIN1, NINJURIN) gene (Gene ID 4814) in human. Ninjurin-1 (NINJ1) is a cell surface adhesion molecule originally identified to be up-regulated in neuronal and Schwann cells after sciatic nerve injury. NINJ1 is also reported to play a role in the progression of multiple sclerosis, an autoimmune inflammatory CNS disease characterized by demyelination and axonal damage. In addition, NINJ1 is known to cause regression of hyaloid blood vessels important for the maturation of the lens during embryonic development. Consistent with the latter role in vascular system, Penile injection of Ninj1-neutralizing antibody is shown to significantly restore erectile function in a murine model of diabetic erectile disfunction (ED) as a result of enhanced cavernous endothelial cell proliferation. Human Ninjurin-1 is a 152-amino aicd protein with boht its N- and C-terminal ends exposed extracellulary (a.a. 1-80, 142-152) and a short intracellular segment (a.a. 102-119) sandwiched between two transmembrane domains (a.a. 81-101, 121-141).
Specificity
Clone 12H6.1 targets the N-terminal extracellular domain of human Ninjurin-1.
Immunogen
Epitope: N-terminal extracellular domain.
GST-tagged recombinant human Ninjurin-1/NINJ1 N-terminal extracellular domain.
Application
Anti-Ninjurin-1 Antibody, clone 12H6.1 is an antibody against NINJ1 for use in Western Blotting, Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected NINJ1 in human liver, pancreas and cerebral cortex tissue sections.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected NINJ1 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected NINJ1 in 10 µg of HeLa cell lysate.
Target description
~17 kDa observed. 16.35 kDa calculated.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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