Anti-TRPM2 Antibody, clone 9D8.1

Transient receptor potential cation channel subfamily M member 2 (EC 3.6.1.13; UniProt O94759; also known as Estrogen-responsive element-associated gene 1 protein, Long transient receptor potential channel 2, LTrpC-2, LTrpC2, Transient receptor potential channel 7, TrpC7) is encoded by the TRPM2 (also known as EREG1, KNP3, LTRPC2, TRPC7, NUDT9H, NUDT9L1) gene (Gene ID 7226) in human. The human TRPM2 is an 1,503-amino acid, six-transmembrane protein with both N- and C-termini exposed intracellularly. TRPM2 contains a pore-forming loop domain located between the fifth and sixth transmembrane region. The N-terminal cytomplasmic region of TRPM2 contains four homologous domains with unknown function and a calmodulin-/CaM-binding IQ-like motif, which is important for channel activation. Its C-terminal cytomplasmic region contains the TRP box, a coiled-coil domain required for the assembly of TRPM2 channels, and an adenosine diphosphate ribose (ADPR) pyrophosphatase domain (Nudix-like domain or NUDT9 homology domain [NUDT9-H]). ADPR binds to the NUDT9-H domain and, in the presence of Ca2+, gates channel opening. TRPM2 activation also results in hydrolysis of ADPR into adenine monophosphate (AMP) and ribose-5-phosphate. TRPM2 is a temperature-sensitive Ca2+-permeable ion channel expressed in various tissues, including brain, lungs, liver, spleen, heart, salivary gland, and pancreas. TRPM2 is involved in redox response, cell proliferation, and insulin secretion by pancreatic β cells. TRPM2 plays a pivotal role in the death of CA1 pyramidal cells and in the development of post-ischemic brain injury. TRPM2 defect is associated with central nervous system disorders such as bipolar disorder type I, amyotrophic lateral sclerosis, Parkinson’s disease, and dementia.

Expected to react with all three spliced isoforms reported by UniProt (O94759).

Epitope: N-terminal cytoplasmic domain.

GST-tagged recombinant protein corresponding to the N-terminal cytoplasmic domain of human TRPM2.

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

Evaluated by Western Blotting in rat hippocampus tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TRPM2 in 10 µg of rat hippocampus tissue lysate.

~170 kDa observed. 171.2 kDa (isoform 1; TRPM2-L), 165.3 kDa (isoform 2), and 95.7 kDa (isoform 3; TRPM2-S) calculated. Uncharacterized band(s) may appear in some lysates.

Mouse monoclonal IgG2aκ in supernatant without preservatives.

Unpurified

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to lot specific datasheet.

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