Anti-VANGL2 Antibody, clone 2G4

Vang-like protein 2 (UniProt Q91ZD4; also known as Loop-tail-associated protein, Loop-tail protein 1, Van Gogh-like protein 2) is encoded by the Vangl2 (also known as Lpp1, Ltap, Stb1, Stbm) gene (Gene ID 93840) in murine species. Mammalian Van Gogh-like proteins (VANGL1 & VANGL2) are orthologues of Vangl/Strabismus originally identified as a core planar-cell-polarity (PCP) gene in Drosophila. VANGL1 & VANGL2 are proteins of the WNT/PCP pathway that acts as a key regulator of cell polarity and directional movements that plays a key role in tissue morphogenesis and embryonic development. PCP is involved in neural tube closure, in the orientation of hair bundle in inner ear sensory cells and of motile cilia in the embryonic node, as well as in asymmetric cell division. VANGL1 and VANGL2 missense mutations have been identified in human embryos affected with severe neural tube defects (NTD). Likewise, homozygous Vangl2 Looptail mutation (Lp) in mice causes morphogenesis and patterning defects in numerous tissues, including the most severe failure in neural tube closure (craniorachischisis). VANGL1 and VANGL2 are both 4-transmembrane (a.a. 109-129, 148-168, 179-199, and 218-238 of murine VANGL2) plasma membrane proteins with cytoplasmic N- and C-terminal ends (a.a. 1-108 and 239-521 of murine VANGL2), they show overlapping, but not identical expression patterns in mouse brain. Both proteins can homo- or heterodimerize, creating different complexes during embryonic development.

Clone 2G4 reacted specifically with VANGL2, but not VANGL1. Clone 2G4 detected shRNA-mediated VANGL2 downregulation in SK-BR-7 human breast carcinoma cells (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).

GST-tagged recombinant VANGL2 N-terminal fragment 100% conserved among human, mouse, and rat species.

Anti-VANGL2 Antibody, clone 2G4, Cat. No. MABN750, is a highly specific rat monoclonal antibody that targets VANGL2 and has been tested in ELISA, Immunofluorescence, Immunoprecipitation, and Western Blotting.

ELISA Analysis: Clone 2G4 hybridoma culture supernatant specifically detected VANGL2, but not VANGL1, N-terminal fragment GST fusion (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).

Immunofluorescence Analysis: A representative lot detected VANGL2 immunoreactivity co-localized with that of VANGL1 at the cell membrane of the stereociliary hair bundles by fluorescent immunohistochemistry staining of 4% paraformaldehyde-fixed cochleae whole mount sections. A drastically reduced VANGL2 was observed in tissue sections from homozygous Vangl2 Looptail mutant (Lp/Lp) mice (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).

Immunoprecipitation Analysis: A representative lot immunoprecipitated VANGL2, but not VANGL1, N-terminal fragment GST fusion, as well as endogenous VANGL2 from untreated, but not VANGL2 shRNA-treated SK-BR-7 human breast carcinoma cells (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).

Western Blotting Analysis: A representative lot detected the endogenous VANGL2 as well as exogenously expressed yellow fluorescent protein Venus-tagged VANGL2 in transfected in MEC1 human chronic lymphocytic leukemia (CLL) cells (Kaucká, M., et al. (2015). Cell Commun Signal. 13:2).

Western Blotting Analysis: A representative lot detected VANGL2, but not VANGL1, N-terminal fragment GST fusion, as well as endogenous VANGL2 in lysate from untreated, but not VANGL2 shRNA-treated SK-BR-7 human breast carcinoma cells (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).

Western Blotting Analysis: A representative lot detected higher VANGL2 expression in mouse brain and lung than kidney. A decreased cochlear VANGL2 expression was found among mice with heterozygous Vangl2 Looptail mutation (Lp), the decrease was even more pronounced among homozygous Vangl2(Lp/Lp) mice (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).

Research Category
Neuroscience

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected VANGL2 in 10 µg of mouse brain tissue lysate.

~60 kDa observed. 59.71 kDa (human) and 59.77 kDa (mouse & rat) calculated. Uncharacterized bands may be observed in some lysate(s).

Format: Purified

Protein G purified.

Purified rat IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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