MABN627
Anti-SDHB Antibody, clone 10C5.1
clone 10C5.1, from mouse
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Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, SDHB, Iron-sulfur subunit of complex II, Ip
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
10C5.1, monoclonal
species reactivity
human, mouse
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SDHB(6390)
General description
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (EC 1.3.5.1; UniProt P21912; also known as Ip, Iron-sulfur subunit of complex II) is encoded by the SDHB (also known as IP, SDH, CWS2, PGL4, SDH1, SDHIP) gene (Gene ID 6390) in human. Originally described by Otto Warburg and now commonly known as the Warburg effect refers to the phenomenon that many tumor cells use aerobic glycolysis instead of mitochondrial respiration for energy production to support cell growth and proliferation. Enzymes involved in the complex network of metabolic pathways are therefore being studied as potential cancer markers and targets for treatment. Succinate dehydrogenase (SDH), also known as mitochondrial respiratory Complex II, is a holoenzyme consisting of four essential subunits, SDHA (a flavoprotein), SDHB (iron-sulfur protein), and two membrane anchor units SDHC and SDHD. SDH assembly requires two factors, SDHAF1 and SDHAF2, while its function is influenced by the deacetylase activity of SIRT3. SDH is the only enzyme complex known to participate in both the TCA cycle and electron transport chain (ETC.). SDH catalyzes the oxidation of succinate in the TCA to form fumarate, a reaction that is coupled to the reduction of ubiquinone to ubiquinol in the ETC. SDH loss-of-function mutations have been linked to pheochromocytoma (PCC), paraganglioma (PGL), gastrointestinal stromal tumor (GIST), renal cell carcinoma, and ovarian cancer.
Immunogen
Epitope: C-terminal half
GST-tagged recombinant protein corresponding to the C-terminal half of human SDHB.
Application
Anti-SDHB Antibody, clone 10C5.1 is an antibody against SDHB for use in Western Blotting, Immunohistochemistry (Paraffin).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected SDHB in 10 µg of Jurkat cell lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected SDHB in human heart, human small intestine, and mouse kidney tissue.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected SDHB in human heart, human small intestine, and mouse kidney tissue.
Quality
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected SDHB in 10 µg of HEK293 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected SDHB in 10 µg of HEK293 cell lysate.
Target description
~30 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
新产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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