MABN624
Anti-Pan-QKI Antibody, clone N147/6
clone N147/6, from mouse
Synonym(s):
Protein quaking, Hqk, HqkI, QKI
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
N147/6, monoclonal
species reactivity
mouse, rat, human
technique(s)
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... QKI(9444)
General description
QKI is also called Protein Quaking or HqkI. QKI is an RNA-binding protein that plays a central role in myelinization. QKI acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation, and is itself, regulated by alternative splicing. QKI is expressed in the frontal cortex of brain, but is shown to be down-regulated in the brain of schizophrenic patients.
~36-40 kDa observed. Uniprot describes six isoforms observed between 35-38 kDa Uncharacterized band(s) may be observed in some cell lysates.
Immunogen
Recombinant protein corresponding to human Pan-QKI.
Application
Anti-Pan-QKI Antibody, clone N147/6 is a highly specific mouse monoclonal antibody & that targets QKI & has been tested in western blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Pan-QKI in human frontal cortex.
Immunohistochemistry Analysis: A representative lot detected Pan-QKI in rat hippocampus tissue (Prof. J. Trimmer, University of California, Davis.).
Western Blotting Analysis: 2 µg/mL from a representative lot detected Pan-QKI in 10 µg of rat brain tissue.
Western Blotting Analysis: 2 µg/mL from a representative lot detected Pan-QKI in 10 µg of mouse brain membrane tissue.
Western Blotting Analysis: A representative lot detected Pan-QKI in RBM cell lysates and in COS cell lysates transfected with QKI-5, QKI-6, QKI-7, and QKI-7b (Prof. J. Trimmer, University of Califorina, Davis.).
Immunohistochemistry Analysis: A representative lot detected Pan-QKI in rat hippocampus tissue (Prof. J. Trimmer, University of California, Davis.).
Western Blotting Analysis: 2 µg/mL from a representative lot detected Pan-QKI in 10 µg of rat brain tissue.
Western Blotting Analysis: 2 µg/mL from a representative lot detected Pan-QKI in 10 µg of mouse brain membrane tissue.
Western Blotting Analysis: A representative lot detected Pan-QKI in RBM cell lysates and in COS cell lysates transfected with QKI-5, QKI-6, QKI-7, and QKI-7b (Prof. J. Trimmer, University of Califorina, Davis.).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Biochem/physiol Actions
This antibody demonstrates cross reactivity against QKI-6, QKI-7, and QKI-7b (Prof. J. Trimmer, University of California, Davis.).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat brain membrane tissue lysate.
Rat brain membrane tissue lysate.
Evaluated by Western Blotting in rat brain membrane tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Pan-QKI in 10 µg of rat brain membrane tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Pan-QKI in 10 µg of rat brain membrane tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Andrew J Best et al.
Nature communications, 15(1), 6328-6328 (2024-07-28)
Disruption of alternative splicing frequently causes or contributes to human diseases and disorders. Consequently, there is a need for efficient and sensitive reporter assays capable of screening chemical libraries for compounds with efficacy in modulating important splicing events. Here, we
Hiroaki Hojo et al.
The Journal of biological chemistry, 295(2), 390-402 (2019-12-04)
MicroRNA-122 (miR-122) is highly expressed in hepatocytes, where it plays an important role in regulating cholesterol and fatty acid metabolism, and it is also a host factor required for hepatitis C virus replication. miR-122 is selectively stabilized by 3' adenylation
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